The Effect of α-Mangosteen on Runt-Related Transcription Factor 2 and Tartrate-Resistant Acid Phosphatase 5b Expressions on Bone Remodeling in Periodontitis (An Experimental Research on Wistar Rats)
- DOI
- 10.2991/ahsr.k.220303.007How to use a DOI?
- Keywords
- periodontitis; α-mangosteen; bone remodeling; RUNX2; TRAP5b
- Abstract
The alteration of supporting structures of the teeth in periodontitis results from the complex interaction between colonies of microorganisms and the immune-inflammatory host response. α-mangosteen contains xanthone which has antioxidant, anti-inflammatory, anti-fungal, and osteoinductive properties and has the potential as an alternative treatment to promote bone remodeling of the damaged bone in periodontitis. This study aims to investigate the effect of α-mangosteen on RUNX2, and TRAP5b. Forty-eight Wistar rats aged 2- 3 months with bodyweight 250-300 g were used in this study. Rats were divided randomly into 8 groups (4 groups in 2-day-periods). Group 1 as the negative control, group 2,3, and 4 were given α- mangosteen hydrogel with the concentration of 0.5%, 1%, and 2% respectively. The alveolar bone on the left mandible of the rats was removed by using low-speed inverted bur with the size of 3mm in the mesiodistal direction, 2mm in the crown-root direction, and 1mm at the buccolingual direction. The α-mangosteen was applied after on the site of the removed bone in the subject groups. At the end of each treatment period (day 7 and day 14), the examination of RUNX2 and TRAP5b expression with immunohistochemical analysis was done. The results of this study showed that on day 7 and day 14, α-mangosteen had an effect on RUNX2. Meanwhile, the TRAP5b examination showed that there was an effect of α-mangosteen on day 7, but not on day 14. α-mangosteen as an antioxidant has a role in bone healing. RUNX2 plays an essential role in osteoblast differentiation, while TRAP5b was found necessary for osteoclast differentiation. The conclusion of this study shows that α-mangosteen affect RUNX2 and TRAP5b.
- Copyright
- © 2022 The Authors. Published by Atlantis Press International B.V.
- Open Access
- This is an open access article under the CC BY-NC license.
Cite this article
TY - CONF AU - Citra Lestari AU - Eryati Darwin AU - Deddi Prima Putra AU - Netti Suharti PY - 2022 DA - 2022/03/08 TI - The Effect of α-Mangosteen on Runt-Related Transcription Factor 2 and Tartrate-Resistant Acid Phosphatase 5b Expressions on Bone Remodeling in Periodontitis (An Experimental Research on Wistar Rats) BT - Proceedings of 1st International Conference on Health Sciences and Biotechnology (ICHB 2021) PB - Atlantis Press SP - 33 EP - 41 SN - 2468-5739 UR - https://doi.org/10.2991/ahsr.k.220303.007 DO - 10.2991/ahsr.k.220303.007 ID - Lestari2022 ER -