Proceedings of the 12th International Conference on Green Technology (ICGT 2022)

Characterization, Antioxidant, and Antibacterial Activity Silver Nanoparticle of Gelidium spinosum

Authors
Evika Sandi Savitri1, *, Eko Budi Minarno1, Lutfiyatul Azizah2
1Biology Study Program, Universitas Islam Negeri Maulana Malik Ibrahim Malang, Malang, Indonesia
2Magister Biology, Universitas Islam Negeri Maulana Malik Ibrahim Malang, Malang, Indonesia
*Corresponding author. Email: evikasandi@bio.uin-malang.ac.id
Corresponding Author
Evika Sandi Savitri
Available Online 29 May 2023.
DOI
10.2991/978-94-6463-148-7_6How to use a DOI?
Keywords
silver nanoparticle; Gelidium spinosum; antioxidant; antibacterial
Abstract

The purpose of this study was to characterize AgNP, and determine antioxidant and antibacterial activities. This research is exploratory descriptive research. The characterization method used PSA (Particle Size Analysis) and FTIR (Fourier Transform Infrared Spectroscopy). The antioxidant activity method uses the DPPH (1.1-diphenyl-2-picrylhydrazyl). The antibacterial activity test used the inhibition zone test with paper disc diffusion (Kirby and Bauer Method), the MIC test used the tube dilution method, and the MBC test used the total plate count method. The results of the research on PSA characterization showed that characterization AgNPs Gelidium spinosum has a size of 107.1 nm, while the FTIR results showed that AgNPs G. spinosum had 7 spectral peaks at wave numbers 466.37 and 534.83(-(CH2)n), 714.11 and 874.27 (C-H aromatic rings), 1035.43 (C-O alcohols, ethers, carboxylic acids, and esters), 1406.24 (C-H alkanes), 1644.42 (C=C alkenes), and 3323.07 (O-H hydrogen bonds in alcohols and phenols). The results of the antioxidant activity of AgNPs Gelidium spinosum an IC50 value of 18.34 ± 5,147 ppm, while the antibacterial inhibition zone test on the sample of AgNPs G. spinosum against E. coli was included in the strong category with an average value of the diameter of the inhibition zone 10.18 mm different from the others sample in the medium category. The results of the minimum inhibitory concentration (MIC) in G. spinosum AgNPs were 18.75 ppm, while the G. spinosum extract was 37.5 ppm. The result of the minimum bactericidal concentration (MBC) on AgNPs G. spinosum was 18.75 ppm, and the extract of G. spinosum is 37.5 ppm. Based on the results of this study AgNPs Gelidium spinosum had strong antioxidant and antibacterial activity against gram-positive and gram-negative bacteria.

Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 12th International Conference on Green Technology (ICGT 2022)
Series
Advances in Engineering Research
Publication Date
29 May 2023
ISBN
978-94-6463-148-7
ISSN
2352-5401
DOI
10.2991/978-94-6463-148-7_6How to use a DOI?
Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

TY  - CONF
AU  - Evika Sandi Savitri
AU  - Eko Budi Minarno
AU  - Lutfiyatul Azizah
PY  - 2023
DA  - 2023/05/29
TI  - Characterization, Antioxidant, and Antibacterial Activity Silver Nanoparticle of Gelidium spinosum
BT  - Proceedings of the 12th International Conference on Green Technology (ICGT 2022)
PB  - Atlantis Press
SP  - 45
EP  - 59
SN  - 2352-5401
UR  - https://doi.org/10.2991/978-94-6463-148-7_6
DO  - 10.2991/978-94-6463-148-7_6
ID  - Savitri2023
ER  -