Proceedings of the 12th International Conference on Green Technology (ICGT 2022)

Endophytic Fungi Isolated from Jasminum sambac L.: Identification, Histological Observation, and Content Analysis of Secondary Metabolites

Authors
Utami Sri Hastuti1, *, Vivi Novianti1, Dwi Rahmawati1, Ria Yustika Sari1, Nadila Sekar Zahida1
1Biology Department, Faculty of Mathematics and Natural Sciences, State University of Malang, Malang, Indonesia
*Corresponding author. Email: tuti_bio_um@yahoo.com
Corresponding Author
Utami Sri Hastuti
Available Online 29 May 2023.
DOI
10.2991/978-94-6463-148-7_4How to use a DOI?
Keywords
endophytic fungi; identification; histological observation; secondary metabolites; Jasminum sambac L.
Abstract

Jasminum Sambac L. plant, especially the flower used for ritual ceremony, i.e.: the wedding ceremony. Commonly medicinal plants have an interaction with endophytic fungi species that lived in their tissues, the interaction form is mutualism symbiotic. The research was done to: (1) identify the endophytic fungi species in the J. sambac flower petal, leaf, and twig tissues; (2) determine the position of endophytic fungi in the J. sambac flower petal, leaf, and twig tissues (3) analyze the contents of secondary metabolite produced by the endophytic fungi liquid cultures. The endophytic fungi mycelium position in the flower petal, leaf, and twig tissues was determined by microscopic observation. The flower petal, leaf, and twigs of J. sambac plant was taken for endophytic fungi isolation and determination their position by microscopic observation. These plant parts cut and inoculated on PDA plate medium. Each endophytic fungi species colonies that grow on PDA plate medium cut into 5x1 cm in size and inoculated in PDB medium, centrifugated and shooked at the speed of 120 rpm at 27 ℃ for a week. The supernatant use for content analysis of tannin, flavonoid, alkaloid, saponin, and terpenoid. The research results are: (1) There are ten endophytic fungi species found in J. sambac tissues: Mycelia sterilia, Colletotrichum kahawae, Chaetomium reflexum, Colletotrichum alienum, Nigrospora gorlenkoawa, Nigrospora oryzae, Cladosporium allienum, Geotrichum candidium, Nigrospora musae, and Alternaria tennuis; (2) the position of endophytic fungi is on the flower petal epidermis cell wall, the leaf neighbor cell wall and stomata guard cell wall, and the twig epidermis and parenchyma cell wall; (3) The secondary metabolites contents of some endophytic fungi are tannin content ranged: 56,09–131,77 mg/kg; flavonoid content ranged: 904,06–1661,88 mg/kg; alkaloid content ranged: 27,34–56,37 mg/kg; terpenoid content ranged: 116,23–222,59 mg/kg; and saponin content ranged: 1, 05–5,63 mg/kg.

Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 12th International Conference on Green Technology (ICGT 2022)
Series
Advances in Engineering Research
Publication Date
29 May 2023
ISBN
978-94-6463-148-7
ISSN
2352-5401
DOI
10.2991/978-94-6463-148-7_4How to use a DOI?
Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

TY  - CONF
AU  - Utami Sri Hastuti
AU  - Vivi Novianti
AU  - Dwi Rahmawati
AU  - Ria Yustika Sari
AU  - Nadila Sekar Zahida
PY  - 2023
DA  - 2023/05/29
TI  - Endophytic Fungi Isolated from Jasminum sambac L.: Identification, Histological Observation, and Content Analysis of Secondary Metabolites
BT  - Proceedings of the 12th International Conference on Green Technology (ICGT 2022)
PB  - Atlantis Press
SP  - 20
EP  - 33
SN  - 2352-5401
UR  - https://doi.org/10.2991/978-94-6463-148-7_4
DO  - 10.2991/978-94-6463-148-7_4
ID  - Hastuti2023
ER  -