How to Measure the Content of Hazardous Substances in Food with the High Performance Liquid Chromatography-Mass Spectrometry Combination Method

DOI

10.2991/icesame-16.2016.28How to use a DOI?

Keywords

HPLC-MS; Food; Hazardous substances; Ethoxyquin antioxidant

Abstract

Purpose: Analyze the results of measuring the content of hazardous substances in food with the high performance liquid chromatography (HPLC)-mass spectrometry (MS) combination method. Method: (1) Establish the HPLC-MS to achieve the one-step extraction of the ethoxyquin antioxidant residue in honey peach and apple by extracting the analyte residue in alkaline sample solution with the help of N-hexane; then analyze it with the C18 chromatographic column and add a certain amount of ammonium acetate into the mobile phase to optimize the peak shape of chromatogram. (2) Establish the HPLC-MS to detect urotropin in food. Results: (1) It is found that the lower limit of detection with the HPLC-MS combination method is 5 g/kg, and the linearity range is between 20-200 g/L; all the recovery rates of different adding levels are between 8.65%-91.9%, and the RSD (relative standard deviation) is between 4.6%-5.4%. (2) For the HPLC-MS combination method, the 0.01mol/L ammonium acetate-acetonitrile is the mobile phase, which provides high accuracy and sensitivity, fits the treatment of a lot of samples, and is used for detecting urotropin to protect the health of consumers. Conclusion: The HPLC-MS combination method is highly feasible in measuring the content of hazardous substances in food.

Copyright

© 2016, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Ying An
PY  - 2016/03
DA  - 2016/03
TI  - How to Measure the Content of Hazardous Substances in Food with the High Performance Liquid Chromatography-Mass Spectrometry Combination Method
BT  - Proceedings of the 2016 International Conference on Education, Sports, Arts and Management Engineering
PB  - Atlantis Press
SP  - 130
EP  - 133
SN  - 2352-5398
UR  - https://doi.org/10.2991/icesame-16.2016.28
DO  - 10.2991/icesame-16.2016.28
ID  - An2016/03
ER  -