Layer-by-layer Assembled Multilayer Membrane with Controllable Amount of Growth Factors for Osteogenic Differentiation

DOI

10.2991/icemct-15.2015.367How to use a DOI?

Keywords

multilayer membrane, layer-by-layer self-assembly, osteogenic differentiation.

Abstract

Creating a bioactive cell-microenvironment and osteogenic functionality interface can be used for the surface modification of bone tissue engineering material, showing a good application prospect. In this paper, a bioactive and osteogenic functionality multilayer membrane with controllable amount of growth factors was fabricated by layer-by-layer assembly technique. Four biological macromolecules (casein, heparin, aidic fibroblast growth factor (aFGF) and collage I) were introduced onto the surface of polyurethane (PU) membrane. Fluorescence intensity analysis and a toluidine blue assay revealed that the successfully multilayer membrane assembly process. The amount of aFGF loaded on membrane was regulated by changing the concentration of heparin, and aFGF can sustainably released from the membrane for 8 days. FDA staining and MTT assay showed that mesenchymal stem cells (hMSCs) on growth factors multilayer membrane fully spread and displayed more obvious pro-liferation behavior. These preliminary results collectively show a practical and simple assembly technique, through which a bioactive and osteogenic material interface can be easily fabricated. This setup has the potential to be used for bone tissue engineering.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Liangting Zhuang
AU  - Zhinan Cao
AU  - Yumin Li
AU  - Yihong Gong
PY  - 2015/06
DA  - 2015/06
TI  - Layer-by-layer Assembled Multilayer Membrane with Controllable Amount of Growth Factors for Osteogenic Differentiation
BT  - Proceedings of the 2015 International Conference on Education, Management and Computing Technology
PB  - Atlantis Press
SP  - 1752
EP  - 1757
SN  - 2352-5398
UR  - https://doi.org/10.2991/icemct-15.2015.367
DO  - 10.2991/icemct-15.2015.367
ID  - Zhuang2015/06
ER  -