Molecular Biological Identification and Determination of Growth Conditions of Facultative CO2 and N2 Fixing Bacterium BHJ
- DOI
- 10.2991/iceesd-18.2018.128How to use a DOI?
- Keywords
- facultative CO2 and N2 fixing bacterium, carbon- and nitrogen-free medium, molecular biology identification, optimum growth conditions.
- Abstract
Currently, the greenhouse effect is becoming increasingly serious, and biological carbon-fixation, especially microbial biological carbon-fixation, can play a unique role in curbing the greenhouse effect．CO2 and N2 are the major greenhouse gases. In this study, a facultative CO2 and N2 fixing bacterium named BHJ (which can fix CO2 and N2 simultaneously) was identified using molecular biology, and its growth conditions were determined. Total DNA from BHJ was extracted and then the 16S rDNA was amplified using polymerase chain reaction. The 16S rDNA sequence was identified and analyzed by alignment with sequences in the GenBank database. Single variables in medium without carbon and nitrogen (KH2PO4 0.2 g, CaCl2 formula: 0.2 g, NaCl 0.2 g, MgSO4 0.3 g, VB 0.0625 g, trace element solution 0.5 mL, distilled water: 1000 mL) was performed. Spectrophotometry was used to measure the optical density changes to determine the optimum temperature, CO2 and N2 ratio, and pH for the growth of BHJ. The 16S rDNA sequence of BHJ showed more than 99% homology with that of Burkholderia cepacia. For the growth of BHJ, the optimum temperature was 34C, the best concentration ratio of CO2 and N2 was 1:99, and the optimum pH was 5.
- Copyright
- © 2018, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Sheng Zhou AU - Xiaoming Wang PY - 2018/05 DA - 2018/05 TI - Molecular Biological Identification and Determination of Growth Conditions of Facultative CO2 and N2 Fixing Bacterium BHJ BT - Proceedings of the 2018 7th International Conference on Energy, Environment and Sustainable Development (ICEESD 2018) PB - Atlantis Press SP - 695 EP - 700 SN - 2352-5401 UR - https://doi.org/10.2991/iceesd-18.2018.128 DO - 10.2991/iceesd-18.2018.128 ID - Zhou2018/05 ER -