Extraction, purification and antibacterial activity of polysaccharides from Purslane

DOI

10.2991/iceesd-17.2017.135How to use a DOI?

Keywords

Purslane polysaccharides, Purification, Antibacterial activity.

Abstract

In this study, the polysaccharides from Purslane were extracted by ultrasonic method and its content was measured by the phenol-sulfuric acid method using glucose as the standard. The crude polysaccharides were collected and purified by DEAE-Cellulose anion-exchange and Sephadex gel-permeation chromatography. Three fractions were obtained and named POL1, POL2 and POL3, respectively. The molecular weights of these three fractions were measured by Sephadex using dextran standard curve. Escherichia coli, Acetic acid bacteria, Saccharomycetes and Aspergillus Niger were used to study the antibacterial activity of the polysaccharides. The results showed that molecular weights of POL1, POL2 and POL3 were 18Kd, 55kd and 108kd, respectively. The polysaccharides had inhibitory effect on microorganisms, and the inhibited order of microorganisms was: Saccharomycetes>Acetic acid bacteria>Aspergillus Niger>Escherichia coli. In addition, the order of antibacterial activities of these three fractions on microorganisms was: POL3>POL2b>POL1b. It could be concluded that three fractions were obtained by purification. These three fractions had inhibitory effect on Saccharomycetes, Acetic acid bacteria, Aspergillus Niger and Escherichia coli. The antibacterial activity of polysaccharides from Purslane against Saccharomycetes was the strongest, while the weakest against Escherichia coli. In addition, POL3 was found to be the most effective against the tested microorganisms among the three fractions.

Copyright

© 2017, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Chunmei Gu
AU  - Shu Pan
PY  - 2017/04
DA  - 2017/04
TI  - Extraction, purification and antibacterial activity of polysaccharides from Purslane
BT  - Proceedings of the 2017 6th International Conference on Energy, Environment and Sustainable Development (ICEESD 2017)
PB  - Atlantis Press
SP  - 734
EP  - 740
SN  - 2352-5401
UR  - https://doi.org/10.2991/iceesd-17.2017.135
DO  - 10.2991/iceesd-17.2017.135
ID  - Gu2017/04
ER  -