Screening citrus SSR molecular marker primers

DOI

10.2991/iceep-18.2018.311How to use a DOI?

Keywords

Citrus；SSR-PCR system；Primer screening；Cluster analysis.

Abstract

Within 38 primer pairs tested, 26 pairs were successfully amplified, and 81 PCR bands were amplified, with an average value of 3.12 bands per primer, 18 pairs showed 100% polymorphic ratio, 2 pairs showed 66.67% polymorphic ratio. In total, 72 PCR polymorphic bands were determined, and in average, 2.77 polymorphic bands were amplified by one primer pair. Twenty primer pairs were screened out, as their PCR bands were distinct, stable and polymorphic. Six citrus materials were clustered via genetic similarity coefficient using 20 pairs of primers screened out,‘Eureka lemon’ and ‘Ichang papeda’ were clustered into one group, and then meet with ‘Ponkan’ and ‘Nanfeng tangerine’. Meanwhile, ‘Gaoban pummelo’ and ‘Kiyomi’ were clustered into another group.

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Xiaojia Wang
AU  - Zhihui Wang
AU  - Siyu Zhang
AU  - Jundou Liu
AU  - Xun Wang
PY  - 2018/09
DA  - 2018/09
TI  - Screening citrus SSR molecular marker primers
BT  - Proceedings of the 2018 7th International Conference on Energy and Environmental Protection (ICEEP 2018)
PB  - Atlantis Press
SP  - 1713
EP  - 1716
SN  - 2352-5401
UR  - https://doi.org/10.2991/iceep-18.2018.311
DO  - 10.2991/iceep-18.2018.311
ID  - Wang2018/09
ER  -