Isolation and characterisation of lead-resistant bacteria from a reverse screen model of simulation soil rice culture

DOI

10.2991/iceep-17.2017.178How to use a DOI?

Keywords

Screening simulated model, 16S rRNA and gyrB, PbRB (Pb-resistant bacteria), bioremediation

Abstract

CGMCC5515(Rifr) were domesticated step by step. Pb-resistant bacteria (PbRB) of CGMCC5515 were selected in directional enrichment and screened with TTC from the pot rice rhizosphere soil with lead and other remediate material, the reverse screen model of simulation soil rice culture with reproducible TTC-detective method could be used as a tool in the R & D of bioremediate agent of lead-polluted soil. The PbRB with lead tolerance 1500 mg L-1 were identified as B. subtilis on the basis of morphological, biochemical and partial sequencing of their 16S rRNA and gyrB genes. Multiple stress tolerant CGMCC5515 immobilizated in alginate beads, formulated with biochar, attapulgite humic acid, reduced the pb of rice seeding to normal level. These results have reinforced the biotechnological potential of CGMCC5515 as a biological control agent.

Copyright

© 2017, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Shengping Xue
AU  - Shengya Meng
AU  - Min Xie
AU  - Yujie Ma
AU  - Lei Liu
AU  - Mengjuan Zhang
PY  - 2017/06
DA  - 2017/06
TI  - Isolation and characterisation of lead-resistant bacteria from a reverse screen model of simulation soil rice culture
BT  - Proceedings of the 2017 6th International Conference on Energy and Environmental Protection (ICEEP 2017)
PB  - Atlantis Press
SP  - 1029
EP  - 1035
SN  - 2352-5401
UR  - https://doi.org/10.2991/iceep-17.2017.178
DO  - 10.2991/iceep-17.2017.178
ID  - Xue2017/06
ER  -