Toxicity Assessment of Silver Nanoparticles using Zebrafish Embryos

DOI

10.2991/iceep-17.2017.85How to use a DOI?

Keywords

biological toxicity, silver nanoparticles, zebrafish embryos

Abstract

In recent years, nanomaterials have attracted great interest from industrial community and researchers due to many reports showing that nanomaterials can be promising vehicles for in vitro diagnosis, in vivo tumor imaging and targeted therapy of many diseases in addition to being wide-spectrum antibacterial agents. Since nanomaterial can readily diffuse into aquatic environments and further enter human body, which probably pose a threat to human health thus limit the real application of nanomaterials in organism. To this end, it is very important to investigate the toxicity of nanomaterial to organisms. Herein, we evaluated the toxicity of two representative silver nanoparticles (AgNPs@PVP, AgNPs@citrate) on the development of zebrafish (a classic model animal) embryos by incubating these silver agents and silver ions (as a positive control) with early embryos at various concentrations for 24, 48, 72 and 96 hours, respectively. The results showed that after 24 hours incubation, Ag+ of 0.2 g/mL led to up to 80 % of mortality of embryos and AgNPs@citrate (~15 nm diameter) can result in death of a small percentage of embryos (mortality of 20 %). However, AgNPs@PVP (~15 nm diameter) did not cause any death of embryos. As the concentration went up to 0.5 g/mL, Ag+ caused 100 % mortality of embryos with 48 hours incubation, and AgNPs@citrate was also highly toxic (mortality of 96 %), but the mortality rate induced by AgNPs@PVP was only 40 %. This data clearly indicate that the AgNPs@PVP is less toxic than AgNPs@citrate and the toxicity of AgNPs@citrate to embryos is comparable to silver ion.

Copyright

© 2017, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Qiaoshu Xu
AU  - Tao Di
AU  - Xin Zhou
AU  - Ning Gu
PY  - 2017/06
DA  - 2017/06
TI  - Toxicity Assessment of Silver Nanoparticles using Zebrafish Embryos
BT  - Proceedings of the 2017 6th International Conference on Energy and Environmental Protection (ICEEP 2017)
PB  - Atlantis Press
SP  - 477
EP  - 482
SN  - 2352-5401
UR  - https://doi.org/10.2991/iceep-17.2017.85
DO  - 10.2991/iceep-17.2017.85
ID  - Xu2017/06
ER  -