Proceedings of the 2016 International Conference on Civil, Transportation and Environment

A specific fluorescence probe for hypochlorous acid based on rhodamin101 thiosemicarbazides and its application for living cells imaging

Authors
Bo-Song Zhu, Zhi-Xiang Han, Tong-Liang Wu, Xiang-Yang Wu
Corresponding Author
Bo-Song Zhu
Available Online January 2016.
DOI
10.2991/iccte-16.2016.195How to use a DOI?
Keywords
Rhodamine 101; Hypochlorous Acid; Bioimaging; Living cells
Abstract

Utilizing the mechanism of HOCl-mediated cyclization of rhodamine 101 thiosemicarbazide (non-fluorescent) to rhodamine 101 oxadiazoles (fluorescent), we successfully developed a novel off-on far-red fluorescent probe (1) toward HOCl with high selectivity, great functionality at physiological pH and good cell-membrane permeability. Probe 1 was linearly proportional to the concentration of HOCl in the range of 1-10 M and the detection limit is 0.3 M. Moreover, the proposed probe (1) was successfully used for the detection of hypochlorous acid in HepG2 cells by fluorescent imaging.

Copyright
© 2016, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 2016 International Conference on Civil, Transportation and Environment
Series
Advances in Engineering Research
Publication Date
January 2016
ISBN
978-94-6252-185-8
ISSN
2352-5401
DOI
10.2991/iccte-16.2016.195How to use a DOI?
Copyright
© 2016, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Bo-Song Zhu
AU  - Zhi-Xiang Han
AU  - Tong-Liang Wu
AU  - Xiang-Yang Wu
PY  - 2016/01
DA  - 2016/01
TI  - A specific fluorescence probe for hypochlorous acid based on rhodamin101 thiosemicarbazides and its application for living cells imaging
BT  - Proceedings of the 2016 International Conference on Civil, Transportation and Environment
PB  - Atlantis Press
SP  - 1115
EP  - 1118
SN  - 2352-5401
UR  - https://doi.org/10.2991/iccte-16.2016.195
DO  - 10.2991/iccte-16.2016.195
ID  - Zhu2016/01
ER  -