Interaction between carminic acid and bovine serum albumin

DOI

10.2991/iccte-16.2016.127How to use a DOI?

Keywords

carminic acid, BSA, fluorescence spectrum, absorption spectrum

Abstract

The binding reaction between carminic acid CA and bovine serum albumin (BSA) was studied by fluorescence and absorption spectrometry. It is shown that there is a powerful ability in CA to quench the fluorescence intensity of BSA, and there is an overlap between the fluorescence spectrum of BSA and the absorption spectrum of CA. The results also indicated that CA could bind with BSA strongly at molar ratio 1 1 and the equilibrium constant were3.0×106. The action distance(r=3.04nm) between donor (BSA) and acceptorcCA was calculated according to Förster s nonradiative energy transfer mechanism. The thermodynamic parameters indicate that CA molecule combines with BSA through the electrostatic force.

Copyright

© 2016, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Feng Wang
AU  - Nan Cao
AU  - Wei Huang
PY  - 2016/01
DA  - 2016/01
TI  - Interaction between carminic acid and bovine serum albumin
BT  - Proceedings of the 2016 International Conference on Civil, Transportation and Environment
PB  - Atlantis Press
SP  - 747
EP  - 750
SN  - 2352-5401
UR  - https://doi.org/10.2991/iccte-16.2016.127
DO  - 10.2991/iccte-16.2016.127
ID  - Wang2016/01
ER  -