Effects of Sulfate Residues on the Interaction of Basic Fibroblast Growth Factor with Heparin Evaluated by Combination of Computer Modeling and Surface Plasmon Resonance methods

DOI

10.2991/iccnce.2013.74How to use a DOI?

Keywords

bFGF, Heparin derivatives, Kinetic Studies, Surface plasmon resonance, Molecular modeling.

Abstract

Surface plasmon resonance (SPR) technology was utilized for kinetic analysis between basic fibroblast growth factor (bFGF) and heparin derivatives in order to quantitatively investigate crucial sites in the sugar chains. The strongest binary interaction was bFGF/DE-6-OS-heparin (KD=1.11nM). Affinity between N-acetyl heparin and bFGF became lower when the amino group was acetylated instead of being sulfated. KD values of bFGF/DE-2-OS-heparin, bFGF/DE-OS-heparin decreased dramatically when O-sulfates on corresponding sites were removed. This was further confirmed by a molecular modeling based on the computer modeling with SYBYL software. From the electrostatic energy Analysis, the binding energy of bFGF/heparin, bFGF/DE-OS-heparin, bFGF/N-acetyl heparin, bFGF/DE-2-OS-heparin, bFGF/DE-6-OS-heparin were -735, -547, -465, -610, -679, respectively. DE-OS-heparin. N-acetyl heparin, DE-2-OS-heparin resulted in more loss of binding capability in compared with the original heparin. It was concluded that SPR technology could be used rapidly, quantitatively, sensitively and within real time to detect molecular process of unknown complex sugar chains/growth factor interactions in combination with the computational molecular modeling in the discovering stage from the complex molecular library.

Copyright

© 2013, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Download article (PDF)

TY  - CONF
AU  - Yapeng Chao
AU  - Zheng Fan
AU  - Jianfen Pei
AU  - Shijun Qian
PY  - 2013/07
DA  - 2013/07
TI  - Effects of Sulfate Residues on the Interaction of Basic Fibroblast Growth Factor with Heparin Evaluated by Combination of Computer Modeling and Surface Plasmon Resonance methods
BT  - Proceedings of the International Conference on Computer, Networks and Communication Engineering (ICCNCE 2013)
PB  - Atlantis Press
SP  - 297
EP  - 300
SN  - 1951-6851
UR  - https://doi.org/10.2991/iccnce.2013.74
DO  - 10.2991/iccnce.2013.74
ID  - Chao2013/07
ER  -