Effects Leaf Ethanol Extract of Graptophyllum pictum L. Griff. to Inhibit Vaginal Atrophy of Menopausal Mouse

DOI

10.2991/absr.k.220406.064How to use a DOI?

Keywords

Graptophyllum pictum; Glycogen; Menopausal; Vagina

Abstract

Menopausal condition with low estrogen level caused vaginal atrophy that it is a condition where vaginal lining become dryer and thinner and it would be easier to get inflammation. So, it was important to find out the treatment to inhibit it. This research was aimed to determine the effects of Graptophyllum pictum (L.) Griff. leaf ethanol extract to inhibit vaginal atrophy of menopausal mice. It used 24 female mice divided into six groups, namely, Group 1, 2 and 3 as control group and group 4,5 and 6 as the treatment groups that were given leaf ethanol extract of Graptophyllum pictum L.Griff. with dozes 10 mg/kg, 20 mg/kg, and 30 mg/kg. Menopausal mice were obtained by doing ovariectomy in mice. Six weeks after the treatment, all mice were anesthetized and dissected to take the vagina for slide preparation staining with PAS staining, IHC for GLTU1 and for measuring the glycogen content. The data were the epithelium thickness and glycogen expression, GLTU 1 expression and glycogen level of menopausal mice vagina. All data were analyzed by Anova test, and the result showed that {Graptophyllum pictum (L.) Griff} leaf ethanol extract effected significantly on the vaginal epithelium thickness and glycogen expression, GLTU1 expression and glycogen level of ovariectomized mice, so it could inhibit vaginal atrophy. The optimal dozes were 10 mg/kg its leaf extract.

Copyright

© 2022 The Authors. Published by Atlantis Press International B.V.

Open Access

This is an open access article distributed under the CC BY-NC 4.0 license.

Download article (PDF)

TY  - CONF
AU  - Listijani Suhargo
AU  - Sri Puji Astuti W.
AU  - Alfiah Hayati
PY  - 2022
DA  - 2022/05/02
TI  - Effects Leaf Ethanol Extract of Graptophyllum pictum L. Griff. to Inhibit Vaginal Atrophy of Menopausal Mouse
BT  - Proceedings of the 7th International Conference on Biological Science (ICBS 2021)
PB  - Atlantis Press
SP  - 460
EP  - 463
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220406.064
DO  - 10.2991/absr.k.220406.064
ID  - Suhargo2022
ER  -