Proceedings of the 7th International Conference on Biological Science (ICBS 2021)

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A Year of COVID-19 Outbreak in Indonesia #2: Variant Development Based on Spike (S) Mutations

Authors
Nicholas Gerry Andreanto1, Dwita Novitasari1, Delia Wahyu Pangesti1, Rizqi Layli Khusufi1, Annasa Sabatia1, Irwansyah Nur Oktafian1, Nur Alfi Maghfirotus Sa’adah1, Reni Krisdayana1, Salsabila Kasta Hygiea Iswara2, Dwi Listyorini1, 2, *
1Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang Jl. Semarang No.5, Malang, 65145, Indonesia
2Department of Biotechnology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang Jl. Semarang No.5, Malang, 65145, Indonesia
*Corresponding author. Email: listyorini.aljabari@um.ac.id
Corresponding Author
Dwi Listyorini
Available Online 2 May 2022.
DOI
10.2991/absr.k.220406.020How to use a DOI?
Keywords
COVID-19; COVID-19 in Indonesia; SARS-CoV-2; Spike gene mutation
Abstract

SARS-CoV-2 has infected millions of people in Indonesia and taken thousands of lives by bonding Spike (S) protein and Angiotensin-Converting Enzyme 2 (ACE2) human cell receptor. Spike gene has a higher mutation rate compared to other genes, which suggested to increase its virulence, transmission and change the virus regulation inside the cell; hence genetic mutation surveillance is needed. This study aimed to determine the dynamics of SARS-CoV-2 spike gene mutation to predict the development of Covid-19 in the future. This study was conducted based on Big-data. Spike gene sequence samples were retrieved from GISAID EpiCoVTM website database and NCBI from March 2020 to March 2021. Multiple alignment of the sequences was achieved using the ClustalW algorithm from BioEdit 7.2.5 version. Mutation and variant analysis, and phylogenetic tree reconstruction, were performed using MEGA X. A total of 146 mutation sites were discovered within Indonesian samples and 100 from 19 comparison countries (Overseas). As many as 135 variants were exclusively found from Indonesian samples and 77 variants from overseas, and 5 from both. One distinct Indonesian variant is thought to have originated from abroad and underwent further mutations in Indonesia. Based on our results, it can be concluded that the SARS-CoV-2 virus is suspected of continuing to mutate if it is still spreading in the community.

Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license.

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Volume Title
Proceedings of the 7th International Conference on Biological Science (ICBS 2021)
Series
Advances in Biological Sciences Research
Publication Date
2 May 2022
ISBN
978-94-6239-573-2
ISSN
2468-5747
DOI
10.2991/absr.k.220406.020How to use a DOI?
Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license.

Cite this article

risenwbib
TY  - CONF
AU  - Nicholas Gerry Andreanto
AU  - Dwita Novitasari
AU  - Delia Wahyu Pangesti
AU  - Rizqi Layli Khusufi
AU  - Annasa Sabatia
AU  - Irwansyah Nur Oktafian
AU  - Nur Alfi Maghfirotus Sa’adah
AU  - Reni Krisdayana
AU  - Salsabila Kasta Hygiea Iswara
AU  - Dwi Listyorini
PY  - 2022
DA  - 2022/05/02
TI  - A Year of COVID-19 Outbreak in Indonesia #2: Variant Development Based on Spike (S) Mutations
BT  - Proceedings of the 7th International Conference on Biological Science (ICBS 2021)
PB  - Atlantis Press
SP  - 131
EP  - 140
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220406.020
DO  - 10.2991/absr.k.220406.020
ID  - Andreanto2022
ER  -