Antioxidant Activity Evaluation of Agarwood Aquilaria malaccensis Lamk. Leaves Extract Using DPPH, FRAP and ABTS Assays

DOI

10.2991/absr.k.220406.004How to use a DOI?

Keywords

Agarwood; Antioxidant; Aquilaria malaccensis Lamk.; Metabolite profiles

Abstract

Indonesia has been known to have large forest areas and rich in biodiversity, which could be the source of primary and secondary metabolites. Along with the technological development and research in medicine, agarwood, which was initially used only for topical body treatments, room fragrances, and religious ceremonies, is started to be investigated for herbal medicines. This study aimed to perform metabolite profiling with spectrophotometry and evaluate the antioxidant activity in agarwood Aquilaria malaccensis Lamk using three assays: DPPH, FRAP and ABTS. The mature leaves used in this study were selected by their though leaf structure and dark green color. The leaves were dried and powdered before being extracted by soxhlet with three different solvents polarity i.e. ethanol, ethyl acetate, and n-hexane. Metabolite profiling was done by scanning the extracts from 200-800 nm. Antioxidant activities were analyzed using 2,2-diphenyl-1-picryhydrazil (DPPH), ethyl benzotiazolin sulfonate (ABTS) and Ferric Reducing Antioxidant Power (FRAP). The result showed that ethanol has the highest yield of extract, followed by ethyl acetate then n-hexane with 10.7%, 8.07%, and 5.23%, respectively. Ethanol contained the highest flavonoid compound compared to the others and has the highest antioxidant activity with IC₅₀ of 37.22 µg/mL using DPPH assay, 66.33 µg/mL using ABTS and 220.188 ± 1.66 µmol TE/g sample using FRAP assays. Therefore, it can be concluded that ethanol extract of Aquilaria malaccensis Lamk. has the highest antioxidant activity and the richest flavonoid compounds compared to the others.

Copyright

© 2022 The Authors. Published by Atlantis Press International B.V.

Open Access

This is an open access article distributed under the CC BY-NC 4.0 license.

Download article (PDF)

TY  - CONF
AU  - Aprilliani Prissilla Halim
AU  - Nastiti Wijayanti
AU  - Lisna Hidayati
AU  - Tri Rini Nuringtyas
PY  - 2022
DA  - 2022/05/02
TI  - Antioxidant Activity Evaluation of Agarwood Aquilaria malaccensis Lamk. Leaves Extract Using DPPH, FRAP and ABTS Assays
BT  - Proceedings of the 7th International Conference on Biological Science (ICBS 2021)
PB  - Atlantis Press
SP  - 18
EP  - 25
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220406.004
DO  - 10.2991/absr.k.220406.004
ID  - Halim2022
ER  -