Anti-Infective Properties of a Sea Cucumber Associated Actinobacteria Kocuria sp. HL 55
- DOI
- 10.2991/absr.k.220406.080How to use a DOI?
- Keywords
- Actinobacteria; Antibiotic; Antiviral; Sea cucumber
- Abstract
“Antibiotics golden era” can end when resistant strains spread immensely. To find new anti-infective agents, we examined sea cucumber associated bacteria isolated from the intestinal part of the echinoderm Holothuria leucospilota. Partial identification using 16S rRNA gene Sanger sequencing revealed Kocuria Flava (99.21% sequence similarity). The ethyl acetate extract from liquid bacterial cultures has a MIC of 4.2, 8.3, and 67 µg/mL against Bacillus subtilis, Staphylococcus aureus, and Eschericia coli, respectively. The extract also showed antiviral activity against Hepatitis C Virus (HCV) with an infectivity value of 77.25 ± 3.13%. The bioactive fraction from the extract showed two peaks from two putative compounds. One peak showed m/z 1140.219 [M+H]+ were identified as putative kocurin. Another peak with m/z 1515.373 [M+H]+ was unidentified. The results showed that Kocuria sp. HL 55, a bacterium associated with H. leucospilota, potentially produces new bioactive compounds. Further analysis for instance extensive 1D and 2D NMR is needed to identify and to characterize the unidentified compound.
- Copyright
- © 2022 The Authors. Published by Atlantis Press International B.V.
- Open Access
- This is an open access article distributed under the CC BY-NC 4.0 license.
Cite this article
TY - CONF AU - Joko Tri Wibowo PY - 2022 DA - 2022/05/02 TI - Anti-Infective Properties of a Sea Cucumber Associated Actinobacteria Kocuria sp. HL 55 BT - Proceedings of the 7th International Conference on Biological Science (ICBS 2021) PB - Atlantis Press SP - 565 EP - 567 SN - 2468-5747 UR - https://doi.org/10.2991/absr.k.220406.080 DO - 10.2991/absr.k.220406.080 ID - Wibowo2022 ER -