Proceedings of the 7th International Conference on Biological Science (ICBS 2021)

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Cytological Analysis of Aerides odorata Lour. from Sleman, Special Region of Yogyakarta

Authors
Kireida Asta Nugraheni1, 2, Febri Yuda Kurniawan1, 3, Anindita Della Rosa Riyadi1, 2, Anggiresti Kinasih1, 2, Hadyan Pratama Lutfi Ilmam1, 2, Endang Semiarti2, *
1Biology Orchid Study Club (BiOSC), Faculty of Biology, Universitas Gadjah Mada 55281 Yogyakarta, Indonesia.
2Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada 55281 Yogyakarta, Indonesia.
3Study Program of Biotechnology, Graduate School, Universitas Gadjah Mada 55281 Yogyakarta, Indonesia.
*Corresponding author. Email: endsemi@ugm.ac.id
Corresponding Author
Endang Semiarti
Available Online 2 May 2022.
DOI
10.2991/absr.k.220406.077How to use a DOI?
Keywords
Aerides odorata; Chromosome; Cytology; Morphology; Sleman
Abstract

Aerides odorata is a species that belongs to the Orchidaceae family and lives as epiphytic orchids. This orchid is distributed across Southeast Asia, India, and China. This orchid occupies one type of habitat. Morphological characters of flowers in this species vary, depending on the habitat occupied. The cytological analysis includes mitotic phase, chromosome number, chromosome morphology, chromosome shape, and karyotyping. Those can be used as cytological data to predict speciation mechanism, phylogenetic analysis, and evolution among species. This research aims to acquire cytological information about flower morphology, stage of mitosis, and karyotype of A. odorata from Sleman, Yogyakarta. In addition, the cytological analysis of this orchid is to compare the number of chromosomes possessed with research that has been done previously. The method in this study consisted of observing the morphological flower characters, chromosome preparation, visualization of mitotic phases, chromosome shape, and karyotyping. The steps in squash consist of fixation with 45% glacial acetic acid, maceration with 1N HCl, staining with Feulgen solution, and squashing. The data were analyzed using Corel Draw 2018 (64 bit), Image Raster 3.0, and Microsoft Office Excel 2019. The orchid flowers found in Sleman, Yogyakarta have various morphology in flower, mainly in color. The phases of mitosis include prophase, prometaphase, metaphase, anaphase, and telophase. This orchid has 38 chromosomes that consisting of 14 metacentric chromosomes and 24 telocentric chromosomes with the karyotype formula obtained is 2n=2x=38=14m+24t.

Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license.

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Volume Title
Proceedings of the 7th International Conference on Biological Science (ICBS 2021)
Series
Advances in Biological Sciences Research
Publication Date
2 May 2022
ISBN
978-94-6239-573-2
ISSN
2468-5747
DOI
10.2991/absr.k.220406.077How to use a DOI?
Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license.

Cite this article

risenwbib
TY  - CONF
AU  - Kireida Asta Nugraheni
AU  - Febri Yuda Kurniawan
AU  - Anindita Della Rosa Riyadi
AU  - Anggiresti Kinasih
AU  - Hadyan Pratama Lutfi Ilmam
AU  - Endang Semiarti
PY  - 2022
DA  - 2022/05/02
TI  - Cytological Analysis of Aerides odorata Lour. from Sleman, Special Region of Yogyakarta
BT  - Proceedings of the 7th International Conference on Biological Science (ICBS 2021)
PB  - Atlantis Press
SP  - 545
EP  - 552
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220406.077
DO  - 10.2991/absr.k.220406.077
ID  - Nugraheni2022
ER  -