Proceedings of the 7th International Conference on Biological Science (ICBS 2021)

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Lipolytic and Proteolytic Activities of Fibrolytic Bacteria from Buffalo (Bubalus bubalis) Rumen

Authors
Siti Lusi Arum Sari1, 2, *, Triyanto Triyanto3, Zuprizal Zuprizal4, Irfan Dwidya Prijambada5, **
1Program of Biotechnology, Graduate Shool, Universitas Gadjah Mada. Jl. Teknika Utara, Pogung, Sleman 55284, Yogyakarta, Indonesia.
2Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret. Jl. Ir. Sutami 36A, Surakarta 57126, Central Java, Indonesia. Tel./fax.: +62-271-663375.
3Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada. Jl. Flora, Bulaksumur, Sleman 55284, Yogyakarta, Indonesia.
4Department of Feed Nutrition, Faculty of Animal Science, Universitas Gadjah Mada. Jl. Flora, Bulaksumur, Sleman 55284, Yogyakarta, Indonesia.
5Department of Agricultural Microbiology, Faculty of Agriculture, Universitas Gadjah Mada. Jl. Flora, Bulaksumur, Sleman 55284, Yogyakarta, Indonesia. Tel.: +62-274-563062, 901290, Fax.: +62-274-563062, 519717
*Corresponding author. Email: sitilusi@staff.uns.ac.id.
**Corresponding author. Email: irfan_prijambada@mail.ugm.ac.id
Corresponding Authors
Siti Lusi Arum Sari, Irfan Dwidya Prijambada
Available Online 2 May 2022.
DOI
10.2991/absr.k.220406.061How to use a DOI?
Keywords
Buffalo rumen; Fibrolytic bacteria; Lipolytic; Proteolytic
Abstract

The use of plant-based feed ingredients in aquaculture is expected to reduce feed costs. However, plant ingredients generally have a low protein content and are difficult to be digested by monogastric animals. The fermentation process is proven to increase the digestibility and nutritional value of plant ingredients. Previous research succeeded in isolating fibrolytic bacteria from buffalo rumen. The ability of microbes to degrade various macromolecules is crucial for their application in feed fermentation. Therefore, this research was conducted to determine the lipolytic and proteolytic activities of fibrolytic bacteria from buffalo rumen. A total of 30 fibrolytic bacterial isolates were tested for their lipolytic and proteolytic activities. The proteolytic activity was determined based on the ability to produce clear zones on the skim milk agar (SMA) medium. Lipolytic activity was determined by using a rhodamine B-olive oil agar medium. The results showed that all fibrolytic bacterial isolates had proteolytic and lipolytic activities. The molecular identification results based on the 16S rRNA gene sequence showed that the bacterial isolates were members of the phylum Firmicutes, namely Bacillus, Paenibacillus and Exiguobacterium; phylum Proteobacteria, namely Acinetobacter, and Klebsiella; and the phylum Bacteroidetes, namely Chryseobacterium.

Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license.

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Volume Title
Proceedings of the 7th International Conference on Biological Science (ICBS 2021)
Series
Advances in Biological Sciences Research
Publication Date
2 May 2022
ISBN
978-94-6239-573-2
ISSN
2468-5747
DOI
10.2991/absr.k.220406.061How to use a DOI?
Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license.

Cite this article

risenwbib
TY  - CONF
AU  - Siti Lusi Arum Sari
AU  - Triyanto Triyanto
AU  - Zuprizal Zuprizal
AU  - Irfan Dwidya Prijambada
PY  - 2022
DA  - 2022/05/02
TI  - Lipolytic and Proteolytic Activities of Fibrolytic Bacteria from Buffalo (Bubalus bubalis) Rumen
BT  - Proceedings of the 7th International Conference on Biological Science (ICBS 2021)
PB  - Atlantis Press
SP  - 437
EP  - 442
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220406.061
DO  - 10.2991/absr.k.220406.061
ID  - Sari2022
ER  -