Prospective Purification and Assay of Thrombolytic Protease from Bacillus sp. HSFI-10 Isolated from Sand Sea Cucumber for Antithrombotic Agent Development

DOI

10.2991/absr.k.220406.057How to use a DOI?

Keywords

Antithrombotic agent; Bacillus sp. HSFI-10; Protease purification; Thrombolytic assays

Abstract

Thrombosis is a cardiovascular disorder due to the formation of a blood clot (thrombus), which can cause a blockage in a blood vessel. Such abnormality is responsible for more than millions of deaths per year in the world. In addition to cardiovascular disease, thrombosis can also occur in patients with the current pandemic era disease, Covid-19. Bacillus sp. HSFI-10 (Holothuria scabra fermented intestine-10) from a previous study has been described as a potential producer of thrombolytic crude protease, which can exhibit competitive thrombolytic activity. However, the purity and other properties of the purified form of the enzyme have not been reported. The remaining challenge is to enable strain HSFI-10 to be used as a source of an effective and low-cost alternative of the thrombolytic agent in the treatment of thrombosis. The purpose of this literature review is to highlight the most appropriate purification method for Bacillus sp. HSFI-10 protease dialysate and to recommend the appropriate assay according to the recommended purification method in the literature. The results suggested that both ion exchange and gel filtration chromatography (IEC and GFC) are the most prospective techniques for the purification of thrombolytic protease. These purification techniques should be carried out as a continuation of the previously concentrated thrombolytic protease of strain HSFI-10 obtained through precipitation using ammonium sulfate followed by dialysis processes. In addition, it was also revealed that the most prospective assays performed on purified proteases to be developed as antithrombotic agents are anticoagulant and platelet aggregation assays. In conclusion, the suggested techniques to purify the dialysate of HSFI 10 are both IEC and GFC. In addition to clot lysis activity assays, it is recommended that anticoagulant and antiplatelet assays are also performed to support the potential of Bacillus sp. HSFI-10 protease as an antithrombotic agent.

Copyright

© 2022 The Authors. Published by Atlantis Press International B.V.

Open Access

This is an open access article distributed under the CC BY-NC 4.0 license.

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TY  - CONF
AU  - Bio Putri Ayanti
AU  - Stalis Norma Ethica
AU  - Ayu Rahmawati Sulisytaningtyas
AU  - Sri Sinto Dewi
AU  - Dewi Seswita Zilda
PY  - 2022
DA  - 2022/05/02
TI  - Prospective Purification and Assay of Thrombolytic Protease from Bacillus sp. HSFI-10 Isolated from Sand Sea Cucumber for Antithrombotic Agent Development
BT  - Proceedings of the 7th International Conference on Biological Science (ICBS 2021)
PB  - Atlantis Press
SP  - 404
EP  - 415
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220406.057
DO  - 10.2991/absr.k.220406.057
ID  - Ayanti2022
ER  -