Proceedings of the 7th International Conference on Biological Science (ICBS 2021)

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Antibacterial Activity and Toxicity Study of Selected Piper Leave Extracts Against the Fish Pathogen (Aeromonas hydrophila)

Authors
Norashikin Anjur1, 2, Siti Fatimah Sabran1, 3, *, Hassan Md Daud4, 5, Nor Zalina Othman6
1Department of Technology and Natural Resources, Faculty of Applied Sciences and Technology (FAST), Universiti Tun Hussein Onn Malaysia (UTHM), Pagoh Educational Hub, 84600 Pagoh, Muar, Johor, Malaysia.
2Tampin Community College, Jalan Impian Nuri 3, Taman Impian Jaya, 73200 Gemencheh, Negeri Sembilan, Malaysia.
3Centre of Research for Sustainable Uses of Natural Resources (CoR-SUNR), Universiti Tun Hussein Onn Malaysia (UTHM), Pagoh Educational Hub, 84600 Pagoh, Muar, Johor, Malaysia.
4Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
5Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
6Innovation Centre In Agritechnology for Advanced BioProcess (ICA), Universiti Teknologi Malaysia, 84600 Pagoh, Educational Hub UTM 2 Street, Johor, Malaysia.
*Corresponding author. Email: fatimahsb@uthm.edu.my
Corresponding Author
Siti Fatimah Sabran
Available Online 2 May 2022.
DOI
10.2991/absr.k.220406.018How to use a DOI?
Keywords
Aeromonas hydrophila; Antibacterial; Bioassay; Brine shrimp; Piper herbs
Abstract

The extensive use of antibiotics in aquaculture has resulted in the emergence of bacterial resistance strains. The medicinal importance of the herb such as Piper betle, Piper sarmentosum, and Piper nigrum evidently proved as one of the most promising commercial botanicals with earlier reported to possess a lot of therapeutic values. Thus, this present study was conducted to evaluate the antibacterial activity of crude aqueous extracts from these three local Piper extracted using the decoction method. The bioassays involved disc diffusion and resazurin microdilution assays against aquacultural pathogenic strain; Aeromonas hydrophila ATCC49140. Further minimum bactericidal concentration (MBC) also was determined. Piper nigrum crude aqueous extract showed the largest inhibition zone (12.71±2.14 mm), followed by P. betle crude aqueous extract (11.79±1.36 mm) and P. sarmentosum (7.33±0.81 mm). The lowest values for minimum inhibitory concentration (MIC) were obtained from P. sarmentosum and P. nigrum crude aqueous extract with the same concentration of 12.5 mg∙mL−1 and the lowest MBC was obtained from P. betle crude aqueous extract (50 mg∙mL−1). In the brine shrimp assay, all crude aqueous Piper extracts of the study demonstrated no toxicity levels. The findings suggested that these three crude aqueous Piper extracts prepared by decoction have the potential to prevent bacterial diseases, particularly in aquaculture. The extract preparation method is pursuing the green technic of plant extraction and is safer to be practiced.

Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license.

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Volume Title
Proceedings of the 7th International Conference on Biological Science (ICBS 2021)
Series
Advances in Biological Sciences Research
Publication Date
2 May 2022
ISBN
978-94-6239-573-2
ISSN
2468-5747
DOI
10.2991/absr.k.220406.018How to use a DOI?
Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license.

Cite this article

risenwbib
TY  - CONF
AU  - Norashikin Anjur
AU  - Siti Fatimah Sabran
AU  - Hassan Md Daud
AU  - Nor Zalina Othman
PY  - 2022
DA  - 2022/05/02
TI  - Antibacterial Activity and Toxicity Study of Selected Piper Leave Extracts Against the Fish Pathogen (Aeromonas hydrophila)
BT  - Proceedings of the 7th International Conference on Biological Science (ICBS 2021)
PB  - Atlantis Press
SP  - 118
EP  - 123
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220406.018
DO  - 10.2991/absr.k.220406.018
ID  - Anjur2022
ER  -