Proceedings of the 4th International Conference Current Breakthrough in Pharmacy (ICB-Pharma 2022)

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Optimization of Peptone Production from Soybeans

Authors
Rochmaningsih1, *, Kun Harismah1, Akida Mulyaningtyas1
1Chemical Engineering, Engineering Faculty, Universitas Muhammadiyah Surakarta, Jl. A. Yani Tromol Pos I, Pabelan, Kartasura, Sukoharjo, 57162, Indonesia
*Corresponding author.
Corresponding Author
Rochmaningsih
Available Online 14 December 2022.
DOI
10.2991/978-94-6463-050-3_25How to use a DOI?
Keywords
Soybean; Peptone; Protein hydrolysis
Abstract

Soybeans are the most popular source of vegetable protein for the people of Indonesia, having protein content ranging from 35%–38%. In the protein analysis of 100 mesh size soybean powder using the Kjeldahl method with the Kjeltec 2100 Distilling Unit, the protein content is 35.95. The high protein content of soybeans can be used for the manufacture of Peptone by hydrolysis process using the papain enzyme. This study used a ratio of 1:2 substrate concentration, 0.3% enzyme concentration, pH 5–7, and a temperature of 40 ℃–60 ℃. The results of this study obtained the highest total dissolved nitrogen value of 14.7264%, at a temperature of 50 ℃, pH of solution 5 and hydrolysis time of 7 h. The total dissolved nitrogen value was compared with the total nitrogen value of the material as a value indicating the result of enzymatic hydrolysis. Peptone-hydrolyzed soybeans are used as an alternative medium for the growth of Escherichia coli ATCC 25922 and Candida albicans ATCC 1023.

Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 4th International Conference Current Breakthrough in Pharmacy (ICB-Pharma 2022)
Series
Atlantis Highlights in Chemistry and Pharmaceutical Sciences
Publication Date
14 December 2022
ISBN
978-94-6463-050-3
ISSN
2590-3195
DOI
10.2991/978-94-6463-050-3_25How to use a DOI?
Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Rochmaningsih
AU  - Kun Harismah
AU  - Akida Mulyaningtyas
PY  - 2022
DA  - 2022/12/14
TI  - Optimization of Peptone Production from Soybeans
BT  - Proceedings of the 4th International Conference Current Breakthrough in Pharmacy (ICB-Pharma 2022)
PB  - Atlantis Press
SP  - 295
EP  - 305
SN  - 2590-3195
UR  - https://doi.org/10.2991/978-94-6463-050-3_25
DO  - 10.2991/978-94-6463-050-3_25
ID  - 2022
ER  -