Comparison of Fixed Range Method and Levey-Jennings Method Used in ELISA Detection Anti-HBc

DOI

10.2991/icaset-18.2018.10How to use a DOI?

Keywords

Fixed range method, Enzyme linked immunosorbent assay, Internal quality control, Levey-Jennings quality control

Abstract

In this paper aims at using the two different methods (fixed range method and Levey-Jennings method) to study the internal quality control of qualitative ELISA detection anti-HBc and exploring the difference, applicability, effectiveness of the two methods. Method -- the author collects the internal quality control data of hepatitis B core antibody during the 16 months (from October 2015 to February 2017). Fixed range method is used. X2 is upper control limit and X1 is lower control limit. Cut-off value adopts one of the lines. ELISA method detection is two half-and-half. If S/CO≥1 is positive, 1 is the lower limit of week positive control product. If S/CO<1 is positive, 1 is upper limit of week positive control product. When the quality control product is stable, X+3SD or X-3SD of S/CO value shall be included into Levey-Jennings quality control chart. If the deviation % is between X1 and X2, it is in control. Otherwise it is out of control. Result -- two groups of data show difference in the two different methods, fixed range method and Levey-Jennings method. The two methods have the similar out-of-control ratio in negative control and positive control. But fixed range method is better than Levey-Jennings method in weak positive control. It has the statistical significance. Conclusion -- when the two methods are used for anti-HBc detection, fixed range method and Levey-Jennings method show difference in weak positive true out-of-control ratio. Fixed range method is more obvious, sensitive and effective.

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Deyi Ju
AU  - Jiandong Wang
AU  - Xiaomei Zhao
PY  - 2018/04
DA  - 2018/04
TI  - Comparison of Fixed Range Method and Levey-Jennings Method Used in ELISA Detection Anti-HBc
BT  - Proceedings of the 2018 8th International Conference on Applied Science, Engineering and Technology (ICASET 2018)
PB  - Atlantis Press
SP  - 49
EP  - 53
SN  - 2352-5401
UR  - https://doi.org/10.2991/icaset-18.2018.10
DO  - 10.2991/icaset-18.2018.10
ID  - Ju2018/04
ER  -