Effect of Methamphetamine on Embryonic Development in Rats

DOI

10.2991/icaset-17.2017.4How to use a DOI?

Keywords

Methamphetamine, Rat, Embryos development, Cleavage

Abstract

To study the effect of methamphetamine (METH) on embryonic development in rats. The number of male SD rats and female SD rats was 50 respectively, male and female rats were taken 30 respectively, which were used to establish the METH chronic poisoning rat model, after that, model animals were randomly divided into 2 groups (n=10). The other 20 male rats and 20 female rats whom were not used for modeling as the control group, which by gender were randomly divided into 2 groups (n=10). Male and female rats were randomly mating in accordance with the ratio of 1:1, whom be divided into four groups: A group (both male and female rats were exposed whom be mated), B group (male exposed rats were mated with female rats whom not be exposed), C group (female exposed rats were mated with male rats whom not be exposed), D group (both male and female rats were not exposed whom be mated, control group). After mated 24h, detection the pregnancy rate of female rats were 98%, 2-cell stage embryos produced by rats of each group were cultured in vitro. There were no significant differences in the number of 2-cell stage embryos produced by rats of each group (P>0.05). The number of 4-cell stage embryos in group A was lower than that in group D (P<0.05).The number of 8-cell stage and 16-cell stage embryos in group A, B and C were significant differences than those in group D (P<0.05 or P<0.01).The number of morula stage and blastula stage embryos in group A, B and C were significant differences than those in group D (P<0.01). Methamphetamine can affects the embryo development of rat, especially have a greater impact to embryos development by both male and female rats were exposed whom be mated.

Copyright

© 2017, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Changlei Li
AU  - Xiji Shu
AU  - Xiaoqing Chen
AU  - Yuwei Liu
AU  - Huiling Yi
AU  - Baomiao Ma
PY  - 2017/05
DA  - 2017/05
TI  - Effect of Methamphetamine on Embryonic Development in Rats
BT  - Proceedings of the 2017 7th International Conference on Applied Science, Engineering and Technology (ICASET 2017)
PB  - Atlantis Press
SP  - 23
EP  - 26
SN  - 2352-5401
UR  - https://doi.org/10.2991/icaset-17.2017.4
DO  - 10.2991/icaset-17.2017.4
ID  - Li2017/05
ER  -