In Vitro and in Vivo Antitumor Efficacy of Berberine-solid Lipid Nanoparticles Against H22 Tumor
- DOI
- 10.2991/icaset-16.2016.64How to use a DOI?
- Keywords
- Solid lipid nanoparticles, Berberine, Antitumor efficacy, H22 tumor
- Abstract
Hepatocarcinoma, a malignant cancer, threaten human life badly. It is a current issue to seek the effective natural remedy from plant to treat cancer due to the resistance of the advanced hepatocarcinoma to chemotherapy. Berberine (Ber), an isoquinoline derivative alkaloid, has a wide range of pharmacological properties and is considered to have anti-hepatocarcinoma effects. However its low oral bioavailability restricts its wide application. In this report, Ber loaded solid lipid nanoparticles (Ber-SLN) was prepared by hot melting and then high pressure homogenization technique. Both in vitro and in vivo anti-hepatocarcinoma effects of Ber-SLN relative to efficacy of bulk Ber were evaluated. The particle size and zeta potential of Ber-SLN were 154.2 ñ 0.8 nm and 18.63ñ0.99mV, respectively. MTT assay showed that Ber-SLN effectively inhibited the proliferation of H22 cells, and the corresponding IC50 values were 11.6 æg/ml (18.3 æg/ml of bulk Ber). In vivo studies also showed higher antitumor efficacy, and inhibition rates was 65.1% (41.4 % of bulk Ber) at 100mg/kg intragastric administration in H22 solid tumor bearing mice. These results suggest that the delivery of Ber-SLN is a promising approach for treating tumors.
- Copyright
- © 2016, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Xiang-ping Meng AU - Yi-fei Wang AU - Zhi-ping Wang PY - 2016/05 DA - 2016/05 TI - In Vitro and in Vivo Antitumor Efficacy of Berberine-solid Lipid Nanoparticles Against H22 Tumor BT - Proceedings of the 2016 6th International Conference on Applied Science, Engineering and Technology PB - Atlantis Press SP - 320 EP - 325 SN - 2352-5401 UR - https://doi.org/10.2991/icaset-16.2016.64 DO - 10.2991/icaset-16.2016.64 ID - Meng2016/05 ER -