Cytotoxicity and Antioxidative Effects of Ethanolic Extract Green and Red Cultivar Ganyong Rhizome (Canna indica Linn) against Colon Cancer Cell Line WiDr

DOI

10.2991/icase-18.2018.3How to use a DOI?

Keywords

Canna Indica; colon cancer; cytotoxicity; WiDr cells; antioxidative

Abstract

Ganyong is a food crops grown in Indonesia. Ganyong rhizome often called ganyong tuber has been used as food by people in antiquity. Now it's the utilization of tuber of this plant as food less interest. Ganyong tuber has a high fibre content, butyric acid and there is some secondary metabolite compounds include: alkaloids, flavonoids, saponins, steroid and triterpenoid, phenolic. The Group of compounds most have activity as antioxidant and anticancer. Colon cancer sufferers in the world occupy third place, and will expected to go up in number due to dietary changes and less unhealthy activity. Ganyong who grew up in indonesia is composed of two varieties red and green ganyong and differences between these two varieties have not been much studied. The aimed of this research to know the cytotoxicity and antioxidative effect of ethanolic extract tuber ganyong either red or green cultivar against WiDr colon cancer cell line. Method : Cytotoxicity activity was studied with MTT assay and antioxidative activity used DPPH assay. Rhizome ganyong extracts tested from two cultivar of red and green with ethanol solvent. Variation concentration used in cell line test at (ppm) 2000; 1500; 1000; 750; 500; 250. L-ascorbid acid used standart in DPPH assay. Cell viability and absorbance data analyzed by probit analysis to get the values of Ic50. The research results obtained Ic50 values of extract ethanolic Red ganyong 1031,75 + 177 ppm of ethanol extract and green ganyong 829 + 130 ppm. The results of the activity free radical oxidative of ethanol extract ganyong Ic50 value green 593 ppm while the ethanol extract Red 1.112 ganyong ppm. Ethanolic extract green ganyong more inhibits the growth of WiDr cancer cells than red ganyong ethanol extract

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Ms. Ifandari
AU  - Sitarina Widyarini
AU  - L. Hartanto Nugroho
AU  - Rarastoeti Pratiwi
PY  - 2018/10
DA  - 2018/10
TI  - Cytotoxicity and Antioxidative Effects of Ethanolic Extract Green and Red Cultivar Ganyong Rhizome (Canna indica Linn) against Colon Cancer Cell Line WiDr
BT  - Proceedings of the International Conference on Applied Science and Engineering (ICASE 2018)
PB  - Atlantis Press
SP  - 10
EP  - 13
SN  - 2352-5401
UR  - https://doi.org/10.2991/icase-18.2018.3
DO  - 10.2991/icase-18.2018.3
ID  - Ifandari2018/10
ER  -