Patterned Photonic Nitrocellulose Membrane for Bio-detection Based on Coffee-Ring Effect
- DOI
- 10.2991/icammce-18.2018.1How to use a DOI?
- Keywords
- Analytical Chemistry; Photonic crystal, Fluorescence enhancement, Coffee-ring, Nano-imprint, POCT
- Abstract
In this work, we report a method for the fabrication of nitrocellulose (NC) membrane with photonic crystal (PC) pattern for bio-detection. The membrane is prepared by imprinting the corresponding PC pattern into it through thermal Nano-imprint method. Aptamer beacon marked with fluorescein is used for detection of target molecules. Only one droplet (2-3μl) containing the aptamer-target complexes is dripped onto the nitrocellulose membrane. After evaporation (15-20min), the droplet is completely dried and the complexes form a coffee ring on the membrane. The ring keeps growing with the increase of complexes concentration.As the aptamer beacon is marked with fluorescein and PC pattern has a fluorescence enhancement effect, the fluorescent signal on the coffee ring for detection is considerable improved. Fluorescent detection of adenosine triphosphate (ATP) is used for testing the application of this method. Owing to fluorescence enhancement effect of PC, the fluorescent signal for detection is improved and the limit of detection is 80.2μM for ATP. Therefore, we believe this method is promising for the fabrication of patterned photonic nitrocellulose membrane for bio-detection and point-of-care testing.
- Copyright
- © 2018, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Litianyi Tang AU - Hong Liu PY - 2018/05 DA - 2018/05 TI - Patterned Photonic Nitrocellulose Membrane for Bio-detection Based on Coffee-Ring Effect BT - Proceedings of the 2018 3rd International Conference on Advances in Materials, Mechatronics and Civil Engineering (ICAMMCE 2018) PB - Atlantis Press SP - 1 EP - 5 SN - 2352-5401 UR - https://doi.org/10.2991/icammce-18.2018.1 DO - 10.2991/icammce-18.2018.1 ID - Tang2018/05 ER -