Proceedings of the 2015 International Conference on Advanced Engineering Materials and Technology

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Study on uptake of PLA-Pluronic P85-PLA nanoparticles with Caco-2 cells

Authors
Cheng Chuang Shu, Han Huang, Lihua Yao, Li Zhen Sun, Xiang Yuan Xiong, Yu Ping Li
Corresponding Author
Cheng Chuang Shu
Available Online August 2015.
DOI
10.2991/icaemt-15.2015.114How to use a DOI?
Keywords
PLA-Pluronic P85-PLA; nanoparticle; insulin; Caco-2 cell; uptake
Abstract

We have shown that insulin-loaded Poly(lactic acid)-b-Pluronic-b-poly(lactic acid) nanoparticles (PLA-P85-PLA-NPs) had the hypoglycemic effect after oral administration on diabetic mice. In the present work, free insulin (INS) as controls, the influence of some factors such as time, temperature, pH level, and INS concentration on the uptake efficacy of PLA-P85-PLA-NPs as drug carriers were investigated through a Caco-2 cells. The micrographs of Caco-2 cell model were observed by microscopy. The cytotoxicity of PLA-P85-PLA-NPs in vitro was evaluated by MTT assay. The insulin concentrations in Caco-2 cells were determined by high-performance liquid chromatography (HPLC). Compared with INS groups, PLA-P85-PLA-NPs uptake was not controlled by pH levels. PLA-P85-PLA-NPs can be uptake by Caco-2 cells, and the absorption of PLA-P85-PLA-NPs is dependent on the concentration and incubation time. INS-loaded PLA-P85-PLA-NPs significantly enhanced insulin absorption. These results suggest that PLA-P85-PLA-NPs were more efficient than individual drugs into Caco-2 cells in a short period of time, and PLA-P85-PLA-NPs might be a useful drug carrier for proteins and peptides.

Copyright
© 2015, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 2015 International Conference on Advanced Engineering Materials and Technology
Series
Advances in Engineering Research
Publication Date
August 2015
ISBN
978-94-6252-108-7
ISSN
2352-5401
DOI
10.2991/icaemt-15.2015.114How to use a DOI?
Copyright
© 2015, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

risenwbib
TY  - CONF
AU  - Cheng Chuang Shu
AU  - Han Huang
AU  - Lihua Yao
AU  - Li Zhen Sun
AU  - Xiang Yuan Xiong
AU  - Yu Ping Li
PY  - 2015/08
DA  - 2015/08
TI  - Study on uptake of PLA-Pluronic P85-PLA nanoparticles with Caco-2 cells
BT  - Proceedings of the 2015 International Conference on Advanced Engineering Materials and Technology
PB  - Atlantis Press
SP  - 591
EP  - 596
SN  - 2352-5401
UR  - https://doi.org/10.2991/icaemt-15.2015.114
DO  - 10.2991/icaemt-15.2015.114
ID  - Shu2015/08
ER  -