Structure-antimicrobial activity relationship and action mechanism of dimeric quaternary ammonium amphiphiles

DOI

10.2991/ic3me-15.2015.82How to use a DOI?

Keywords

Dimeric quaternary ammonium, Antimicrobial, Escherichia coli.

Abstract

The antibacterial activity of the five dimeric quaternary ammonium amphiphiles against Escherichia coli were evaluated by determining the minimum inhibitory concentration (MIC) and compared with one monomeric quaternary ammonium amphiphile. The antibacterial activity of dimeric amphiphiles is superior to the corresponding monomeric amphiphile. The action mechanism was investigated by measuring the activity of -galactosidase from E. coli and scanning electron microscopy (SEM) imaging of E. coli cells. The result of -galactosidase activity shows that 12-8-12 disrupts the membrane of E.coli leading to the releasing of intracellular contents and death of bacterial cells. This conclusion was supported by scanning electron microscopy, which imaged the holes on the cell membrane and irregular shape of cells caused by treatment of 12-8-12.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Xiao Fang Zhao
AU  - Ya Hong Li
AU  - Hao Yuan
PY  - 2015/08
DA  - 2015/08
TI  - Structure-antimicrobial activity relationship and action mechanism of dimeric quaternary ammonium amphiphiles
BT  - Proceedings of the 3rd International Conference on Material, Mechanical and Manufacturing Engineering
PB  - Atlantis Press
SP  - 417
EP  - 420
SN  - 2352-5401
UR  - https://doi.org/10.2991/ic3me-15.2015.82
DO  - 10.2991/ic3me-15.2015.82
ID  - Zhao2015/08
ER  -