Antioxidant activity of collagen hydrolysate obtained from tilapia scales in vitro and in vivio assays
- DOI
- 10.2991/ic3me-15.2015.59How to use a DOI?
- Keywords
- Tilapia scale; collagen hydrolysate; antioxidant
- Abstract
Collagen hydrolysate of S and C were prepared by enzymatic hydrolysis decalcified tilapia scale and pepsin-solubilised collagen extracted from decalcified tilapia scale. The antioxidative activity of C and S were evaluated by their effects on scavenging ability of superoxide anion O2- radicals, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and reductive ability in vivo. Besides, the activity of glutathione peroxidase (GSH-Px), total antioxidant capacity (T-AOC) and content of malondialdehyde (MDA) in vitro of the laboratory mouse were detected. The results indicated that collagen hydrolysate of C and S show prominent inhibitory effect on scavenging O2- with the fifty-percent inhibitory concentration (IC50) of 3.7 and 6.5 mg/mL, respectively. The IC50 of collagen hydrolysate of C and S inhibit the DPPH were 8.4 and 7.2 mg/mL, respectively. Two collagen hydrolysates demonstrated highly reducing activity. In vivo experimentation suggested that C and S can significantly improve the GSH-Px activity, T-AOC and reduce the contents of MDA. As a consequence of results, collagen hydrolysates of C and S extracted from decalcified tilapia scale have a good antioxidation.
- Copyright
- © 2015, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Shaokui Zeng AU - Wenlong Wu AU - Chaohua Zhang AU - Juanjuan Yin AU - Zhenlin Li PY - 2015/08 DA - 2015/08 TI - Antioxidant activity of collagen hydrolysate obtained from tilapia scales in vitro and in vivio assays BT - Proceedings of the 3rd International Conference on Material, Mechanical and Manufacturing Engineering PB - Atlantis Press SP - 311 EP - 315 SN - 2352-5401 UR - https://doi.org/10.2991/ic3me-15.2015.59 DO - 10.2991/ic3me-15.2015.59 ID - Zeng2015/08 ER -