The analysis of T-DNA insertional Colletotrichum gloeosporioides in Stylo pathogenicity-weakened mutant strain 1681

DOI

10.2991/ic3me-15.2015.43How to use a DOI?

Keywords

Stylosanthes guianensias; Colletotrichum gloeosporioides; T-DNA; pathogenicity

Abstract

Biological phenotypes of mutant strain 1681 that was the pathogenicity-reduced strain in Colletotrichum gloeosporioides of Stylosanthes guianensia were analyzed by testing growth rate, colonial morphologies and sporulation ability. TAIL-PCR was used to identify the T-DNA integration site and the genes of flanking right site of the T-DNA. The sequences was analyzed by local blast and the gene and the function were predicted by bioinformatics methods. The results showed that the pathogenicity of the mutant 1681 was reduced by comparing with wild type strain CH008 in Colletotrichum gloeosporioides of Stylosanthes guianensis. The growth rate between strain 1681(1.42±0.02)cm/d and CH008(1.41±0.03)cm/d had no significant difference. The sporulation between strain 1681(1.50±0.25)×106 /mL and CH008(9.26±0.14)×106/mL had significant difference. The spore germination rate between strain 1681(0.00±0.00)% and CH008(91.14±6.66)% had significant difference. In contrast the colony diameter and colony morphology had no significant difference. Two sequences were cloned by TAIL-PCR. The length of the RB flanking sequence was 412 bp and the LB flanking sequence was 381 bp. The blast result showed that the sequence had 100% homology to genome of wild type strain CH008 in Colletotrichum gloeosporioides of Stylosanthes guianensis. The bioinformatics analysis showed the T-DNA maker the area of initial exon. It contains a complete open reading frame (ORF), encoding 682 amino acid. By predicting the function of sequence in NCBI, the blast result showed that the sequence had 99% homology to gene Nara gc5 phenylalanyl-tRNA synthetase (PheRS) beta chain core domain. It belongs to the class II aminoacyl-tRNA synthetases (aaRS) like-core super family based upon its structure.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Peidong Xu
AU  - Xiaolan Zheng
AU  - Wen Tang
AU  - Qiujie Li
AU  - Weihuai Wu
AU  - Jingen Xi
AU  - Yanqiong Liang
AU  - Jinlong Zheng
AU  - Rui Li
AU  - Chicheng Zhang
AU  - Kexian Yi
AU  - Chunping He
AU  - Xiaobo Zhang
PY  - 2015/08
DA  - 2015/08
TI  - The analysis of T-DNA insertional Colletotrichum gloeosporioides in Stylo pathogenicity-weakened mutant strain 1681
BT  - Proceedings of the 3rd International Conference on Material, Mechanical and Manufacturing Engineering
PB  - Atlantis Press
SP  - 222
EP  - 230
SN  - 2352-5401
UR  - https://doi.org/10.2991/ic3me-15.2015.43
DO  - 10.2991/ic3me-15.2015.43
ID  - Xu2015/08
ER  -