Proceedings of the 6th International Conference of Food, Agriculture, and Natural Resource (IC-FANRES 2021)

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The Mechanistic Study on the Effect of Acacia concinna and Cymbopogon nardus on Lipid Metabolism

Authors
Wijitrapha Ruangaram1, *, Eisuke Kato2
1Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan,wijit@chem.agr.hokudai.ac.jp
2Division of Fundamental AgriScience and Research, Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan,eikato@chem.agr.hokudai.ac.jp
*Corresponding author. Email: wijit@chem.agr.hokudai.ac.jp
Corresponding Author
Wijitrapha Ruangaram
Available Online 5 January 2022.
DOI
10.2991/absr.k.220101.004How to use a DOI?
Keywords
Lipid metabolism; Mechanistic study; Thai medicinal plants
Abstract

Obesity is one of the most concerning health problems globally. At the moment, medicinal plants have been widely studied in order to assist in the treatment of obesity instead of the developed drugs. From our previous study, Thai medicinal plants were tested through screening methods regarding anti-obesogenic activity and several candidates were selected. Subsequently, in this study, 2 of the candidate plants naming Acacia concinna (Wild.) DC., and Cymbopogon nardus (L.) Rendle were selected and studied in more detail on the mechanistic aspect related to lipid metabolism. The plants were extracted in 50% (v/v) aq. methanol and resuspended in 10% DMSO to be tested in 3T3-L1 adipocytes and NCTC 1469 hepatocytes. The cells were treated with the plant extract and the effect on expression of lipid metabolism related genes were examined using RT-qPCR. The target genes related to lipid metabolism are listed as following: adipogenesis (PPARγ, C/EBPα), lipogenesis (FAS, ACC-1, SREBP-1), lipolysis (HSL, ATGL, perilipin-1) and fatty acid β-oxidation (PPARα). In 3T3-L1 adipocytes, A. concinna plants downregulated adipogenesis genes, lipogenesis genes, and lipolytic genes. On the other hand, C. nardus has a difference in some genes such as upregulated C/EBPα and no significant changes in ATGL. Furthermore, In NCTC 1469 hepatocytes, 2 plants acted distinctively in each gene. A. concinna lowered all the target gene expressions with some being significantly different but some were not. C. nardus downregulated gene expression of C/EBPα, FAS, SREBP-1, HSL, ATGL and PPARγ but no significant changes were observed in ACC-1 and PPARγ. A. concinna and C. nardus might have potential to combat obesity through improving the lipid metabolism, which can act as anti-adipogenic and anti-lipogenic agents. Further study such as in vivo study will be conducted to strengthen the data.

Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article under the CC BY-NC license.

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Volume Title
Proceedings of the 6th International Conference of Food, Agriculture, and Natural Resource (IC-FANRES 2021)
Series
Advances in Biological Sciences Research
Publication Date
5 January 2022
ISBN
978-94-6239-506-0
ISSN
2468-5747
DOI
10.2991/absr.k.220101.004How to use a DOI?
Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article under the CC BY-NC license.

Cite this article

risenwbib
TY  - CONF
AU  - Wijitrapha Ruangaram
AU  - Eisuke Kato
PY  - 2022
DA  - 2022/01/05
TI  - The Mechanistic Study on the Effect of Acacia concinna and Cymbopogon nardus on Lipid Metabolism
BT  - Proceedings of the 6th International Conference of Food, Agriculture, and Natural Resource (IC-FANRES 2021)
PB  - Atlantis Press
SP  - 22
EP  - 26
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220101.004
DO  - 10.2991/absr.k.220101.004
ID  - Ruangaram2022
ER  -