Analysis of Lipase and Lipid Formation in Adipocytes Inhibitory Capabilities in Kelakai (Stenochlaena palustris) for Obesity Management

DOI

10.2991/aer.k.200325.044How to use a DOI?

Keywords

S. palustris, obesity management, lipase, 3T3-L1 adipocytes, fractionation

Abstract

The objective was to test S. palustris which has high antioxidant activity for bioactivities that may alleviate obesity: lipase inhibitory activity and lipid accumulated in 3T3-L1 adipocytes. Water, 50% methanol and methanol extracts of S. palustris were tested for lipase inhibitory activity and lipid accumulation inhibition in 3T3-L1 adipocytes. For lipase inhibitory activity, the extracts and fractions with highest activity were separated and assayed again. Fractionation through column chromatography was done on the water extract to separate it into water, 50% methanol and methanol fractions. The 50% methanol and methanol fractions from the first column were fractionated again with a different column into water, 20%, 40%, 60%, 80% and methanol fractions. The result was the water extract showed the highest lipase inhibition while the methanol extract showed the highest lipid accumulation inhibition in adipocytes. In conclusion, S. palustris extracts with its lipase inhibitory activity as well as lipid accumulation in adipocytes suppression activity is promising for future research aimed at obesity management.

Copyright

© 2020, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Download article (PDF)

TY  - CONF
AU  - Maria D.P.T. Guanwan Puteri
AU  - Filiana Santoso
AU  - Evelyn Antoinette Halim
AU  - Della Rahmawati
AU  - Eisuke Kato
AU  - Yanetri Asi Nion
PY  - 2020
DA  - 2020/03/30
TI  - Analysis of Lipase and Lipid Formation in Adipocytes Inhibitory Capabilities in Kelakai (Stenochlaena palustris) for Obesity Management
BT  - Proceedings of the 5th International Conference on Food, Agriculture and Natural Resources (FANRes 2019)
PB  - Atlantis Press
SP  - 226
EP  - 231
SN  - 2352-5401
UR  - https://doi.org/10.2991/aer.k.200325.044
DO  - 10.2991/aer.k.200325.044
ID  - GuanwanPuteri2020
ER  -