In Vitro Bioassay of Allelopathy in Robusta Coffee Callus Using Sandwich Method
- DOI
- 10.2991/aer.k.200325.030How to use a DOI?
- Keywords
- allelopathy, coffee callus, sandwich method
- Abstract
The allelopathic potential of various plants, including coffee species, has been studied. Although the allelopathic potential of coffee leaves, stems, and roots has been reported, there is no detailed information about coffee callus. Since this callus produces bioactive metabolites, we focused on investigating the allelopathic potential of Robusta coffee (Coffea canephora) callus as follows. The sandwich (SW) method, a small-scale bioassay using dried leaves of test plants and recipient lettuce seedlings, was adapted to identify the allelopathic activity of target coffee calli. Test samples were prepared as 10 mg of dried calli under five drying conditions: oven drying at 40 °C, 60 °C, 80 °C, silica gel drying, and freeze-drying. The appearance of each dried callus and chemical features of its water extract varied depending on drying conditions. However, all Robusta coffee calli showed a strong suppressive effect on the growth of hypocotyls and roots of lettuce seedlings. Based on this finding, we analyzed the endogenous level of caffeine (1,3,7-trimethylxanthine), a known allelochemical of coffee and tea plants, using HPLC. In this report, we discuss 1) technical points such as moisture content, drying method, and managing sample quality in the SW method for calli, and 2) the relationship between allelopathic potential and endogenous caffeine level of Robusta coffee calli.
- Copyright
- © 2020, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Muchamad Imam Asrori AU - Hamako Sasamoto AU - Shinjiro Ogita PY - 2020 DA - 2020/03/30 TI - In Vitro Bioassay of Allelopathy in Robusta Coffee Callus Using Sandwich Method BT - Proceedings of the 5th International Conference on Food, Agriculture and Natural Resources (FANRes 2019) PB - Atlantis Press SP - 147 EP - 151 SN - 2352-5401 UR - https://doi.org/10.2991/aer.k.200325.030 DO - 10.2991/aer.k.200325.030 ID - Asrori2020 ER -