Antioxidant Activity of Protein Hydrolysates Derived from Javanese Freshwater Fishes

DOI

10.2991/fanres-18.2018.44How to use a DOI?

Keywords

Amino Acid, Antioxidant Activity, Javanese Freshwater Fishes, Protein Hydrolysates.

Abstract

Fish protein hydrolysates have been identified as the rich source of bioactive peptides. Thus, this study aims to determine the antioxidant activity of protein hydrolysates derived from common barb (Rasbora jacobsoni) and java carp (Barbonymus gonionotus). Therefore, in this study, two types of freshwater fishes were evaluated as a raw material to obtain protein hydrolysates exhibiting antioxidant activity. The fish’s protein was hydrolyzed by biduri protease with a concentration of 1, 2 and 3% (v/w) for 3 hours. Afterwards, the resulted dry hydrolysates were observed to determine the degree of hydrolysis, antioxidant activity (DPPH and reducing power), amino acid composition, and molecular weight. The result shows that the protein hydrolysates derived from common barb and java carp fish had percent value of inhibition (%RSA) respectively of 42.70% and 53.21%, an IC50 value of 1159.76 ppm and 935.84 ppm, reducing power value of 0.508 and 0.524. The protein hydrolysates derived from java carp fish had 15 kinds of an amino acid such as glutamic acid, aspartic acid and lysine, respectively of 13.34%; 8.36%; and 8.20%. The molecular weight distribution of protein hydrolysates derived from java carp fish had a value of about 23.09 kDa; 59.08 kDa

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Yuli Witono
AU  - Iwan Taruna
AU  - Maryanto Maryanto
AU  - Riska Rian Fauziah
AU  - Wulan Suci Wahyuningtyas
PY  - 2018/11
DA  - 2018/11
TI  - Antioxidant Activity of Protein Hydrolysates Derived from Javanese Freshwater Fishes
BT  - Proceedings of the International Conference on Food, Agriculture and Natural Resources (FANRes 2018)
PB  - Atlantis Press
SP  - 213
EP  - 217
SN  - 2352-5401
UR  - https://doi.org/10.2991/fanres-18.2018.44
DO  - 10.2991/fanres-18.2018.44
ID  - Witono2018/11
ER  -