Immobilization of glucoamylase on stimuli-sensitive macroporous semi-interpenetrating stimuli-sensitive hydrogel carriers

DOI

10.2991/eesed-16.2017.19How to use a DOI?

Keywords

Immobilization; Glucoamylase; Stimuli-sensitive hydrogel carriers; Thermo-/light-sensitive

Abstract

Based on the special phase transition property of copolymer hydrogel of N-is opropylacrylamide, the immobilization of glucoamylase on five macro porous semi-interpenetrating stimuli-sensitive hydrogel carriers was studied. Four good preparations were screened, i.e. GelNIPAm/PAEMA-2.5, Gel NAEP/PAEMA-2.5, Gel NAP/PAEMA-2.5 and Gel NDEP/PAEMA-2.5, the immobilization yield øCactivity) of them were 11.4%, 12.7%, 14.9% and 15.3% respectively under optimal conditions. The most preferred temperature of these immobilized glucoamylase was 30 øC, and the adapted pH was about pH 4.5. The activities of the latter three photo-sensitive preparations could be adjusted by UV irradiation during their phase transition. After month storage, the activity retention rate was no less than 80% for every one preparations cross-linked with 2.5 vol % of glutaraldehyde.

Copyright

© 2017, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Li-Xia Li
AU  - Yi Yang
AU  - Fang Zhu
AU  - Xiang-Yang Wu
PY  - 2016/08
DA  - 2016/08
TI  - Immobilization of glucoamylase on stimuli-sensitive macroporous semi-interpenetrating stimuli-sensitive hydrogel carriers
BT  - 2nd Annual International Conference on Energy, Environmental & Sustainable Ecosystem Development (EESED 2016)
PB  - Atlantis Press
SP  - 145
EP  - 155
SN  - 2352-5401
UR  - https://doi.org/10.2991/eesed-16.2017.19
DO  - 10.2991/eesed-16.2017.19
ID  - Li2016/08
ER  -