Thermal Simulation for Continuous-flow PCR System

DOI

10.2991/cset-16.2016.64How to use a DOI?

Keywords

PCR, microfluidic, COMSOL, Simulation

Abstract

A continuous-flow PCR system is presented, which based on the microfluidic platform briefly consists of a PMMA chip with a PCR liquid channel, three heating units. Each of them contained a heat conducting block on a commercially available thin PI substrate. In order to ensure temperature uniformity across the PCR temperature zones and minimal cross-talk between the zones, the design of chip and heating units was assisted by COMSOL. The simulation results presented the most suitable thickness for the chip and heat conducting block as well as the height limit for the liquid channel. Within the optimized parameters, our system not only has the advantages of fast amplification rates, but also has the benefits of small thermal mass and low power consumption.

Copyright

© 2016, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Shenglan Zeng
AU  - Bingyu Chen
AU  - Lingfeng Cai
AU  - Quanjun Liu
PY  - 2016/08
DA  - 2016/08
TI  - Thermal Simulation for Continuous-flow PCR System
BT  - Proceedings of the 2016 International Conference on Computer Science and Electronic Technology
PB  - Atlantis Press
SP  - 273
EP  - 276
SN  - 2352-538X
UR  - https://doi.org/10.2991/cset-16.2016.64
DO  - 10.2991/cset-16.2016.64
ID  - Zeng2016/08
ER  -