Simultaneous Quantitation of Organic Acids and Monosaccharides by High-Performance Liquid Chromatography

DOI

10.2991/cmfe-15.2015.89How to use a DOI?

Keywords

organic acid; monosaccharides; simultaneous quantitation; fruit? beverage

Abstract

Objective: a fast, simply, reliably and accurately high-performance liquid chromatography (HPLC) method for the simultaneous quantitation of commonly used organic acid and monosaccharides in food industry such as benzoic, malic, citric and quinic acid, fructose, glucose has been developed. Methods: In order to obtain a satisfactory separation in a short elution time, various HPLC operational factors like composition of mobile phase, column temperature and flow rate affecting analysis had been optimized. Results: The optimum determination conditions are as follows: for a CAPCELL PAK NH2 column, flow rate of 1 mL/min, 75% acetonitrile (pH 2.25 adjusted by phosphoric acid) as mobile phase, column temperature of 35 oC. Under these conditions, this method could achieve adequate separation within 18 min and show the excellent linearity (R2 > 0.999), high precision (R.S.D < 5%) and good accuracy (The recovery closed to 100%). Moreover, the procedure of this method is very sample, involves little sample preparation which just consists of dilution and filtration before injection, and the cost of this method is relative low compared with ion-exchange HPLC method. Conclusion: it is promising method to simultaneous quantitation of organic acids and monosaccharides in food industry.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Xin Lu
AU  - Hagiwara Shoji
AU  - Guohui Song
AU  - Jinian Huang
AU  - Nabetani Hiroshi
AU  - Ruihong Li
PY  - 2015/07
DA  - 2015/07
TI  - Simultaneous Quantitation of Organic Acids and Monosaccharides by High-Performance Liquid Chromatography
BT  - Proceedings of the International Conference on Chemical, Material and Food Engineering
PB  - Atlantis Press
SP  - 372
EP  - 380
SN  - 2352-5401
UR  - https://doi.org/10.2991/cmfe-15.2015.89
DO  - 10.2991/cmfe-15.2015.89
ID  - Lu2015/07
ER  -