Comparative Research of Magnetic Bead and Traditional Methods to Extract the Genomic DNA of Bean Sprouts

DOI

10.2991/cmfe-15.2015.42How to use a DOI?

Keywords

magnetic bead method; DNA; SDS method ;Young leaves of bean sprouts

Abstract

The Young leaves of green beans sprouts were used as experimental materials, total DNA was extracted by using methods including magnetic bead and traditional SDS methods. Integrality and purity of nucleic acids were detected with agarose gel electrophoresis, ultraviolet absorption and PCR.The results showed that: the total DNA of green beans sprouts extracted by using magnetic beads method had the advantages of high yield and high quality, agarose gel electrophoresis bands is clear and complete, meeting the requirements of the follow-up molecular biology experiments. the total DNA extracted by using SDS method has the characteristics of low production, impurities, easily degradable, agarose gel electrophoresis bands is not clear and complete. Experiments also demonstrated: Compared with SDS method, magnetic beads method to extract DNA had the advantages of simple operation, short time-consuming, stable, high purity, more conducive to rapid DNA detection.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Yanwei Cheng
AU  - Shengnan Kong
AU  - Huiyun Li
AU  - Shuang Han
AU  - Jianming Han
AU  - Xueyi Yang
AU  - Lihui Xiao
AU  - Deguang Du
PY  - 2015/07
DA  - 2015/07
TI  - Comparative Research of Magnetic Bead and Traditional Methods to Extract the Genomic DNA of Bean Sprouts
BT  - Proceedings of the International Conference on Chemical, Material and Food Engineering
PB  - Atlantis Press
SP  - 178
EP  - 181
SN  - 2352-5401
UR  - https://doi.org/10.2991/cmfe-15.2015.42
DO  - 10.2991/cmfe-15.2015.42
ID  - Cheng2015/07
ER  -