Study on Extraction of Polysaccharide by Composite Enzymatic Dilapidating Walls from Waste Wine Yeast Slurry

DOI

10.2991/cmfe-15.2015.31How to use a DOI?

Keywords

enzymatic extraction; cell wall disruption; polysaccharide; yeast slurry ;orthogonal design methodology

Abstract

Objective: To establish a method for the extraction of polysaccharide from waste wine yeast slurry by composite enzymatic method. Methods: On the basis of single factor experiments, the effects of operating conditions, such as enzyme mixture ratio, enzyme added, composite enzymatic extraction pH, and composite enzymatic extraction temperature, were analyzed by orthogonal design methodology. Results: The optimized conditions of composite enzymatic extraction are as follows: the ratio of ?-glucan enzyme to papain enzyme is 3:1, composite enzyme concentration 2.5 g/L, pH value 6.0, temperature 60°C, the polysaccharide yield is 8.97%. Conclusion: The composite enzymatic extraction method has a high extraction rate in extracting the polysaccharide of waste wine yeast slurry. The method is simple and practical.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Download article (PDF)

TY  - CONF
AU  - Yunfeng Hu
AU  - Jing Liang
AU  - Qiuyue Yang
AU  - Ningning Li
PY  - 2015/07
DA  - 2015/07
TI  - Study on Extraction of Polysaccharide by Composite Enzymatic Dilapidating Walls from Waste Wine Yeast Slurry
BT  - Proceedings of the International Conference on Chemical, Material and Food Engineering
PB  - Atlantis Press
SP  - 128
EP  - 131
SN  - 2352-5401
UR  - https://doi.org/10.2991/cmfe-15.2015.31
DO  - 10.2991/cmfe-15.2015.31
ID  - Hu2015/07
ER  -