Extraction and Antioxidant Activity of Polysaccharide from Salvia miltiorrhiza

DOI

10.2991/cmfe-15.2015.26How to use a DOI?

Keywords

Salvia miltiorrhiza; polysaccharides; Ultrasonic extraction; Phenol - sulfuric acid method; antioxidation activity

Abstract

1. Objective: Salvia miltiorrhiza is a traditional Chinese herb medicine and is widely used in clinic application. This study is intended to extract polysaccharides from Salvia miltiorrhiza by ultrasonic method in order to evaluate its antioxidation activity. 2. Methods: (1) Spectrophotometry is used to determine content of polysaccharides from Salvia miltiorrhiza; (2) Ultrasonic method was used to extract polysaccharides from Salvia miltiorrhiza and water as the solution. The effect of the different volume of solvent, extraction time and frequency were conducted (3) The antioxidation of Salvia miltiorrhiza polysaccharides is evaluated by pyrogallol autoxidation and deoxyribose degradation. 3. Results: (1) Phenol - sulfuric acid method was established to test the content of polysaccharide from Salvia miltiorrhiza. The results were in good linear relationship, and Y= 0.9947x+0.0451, R2=0.9996. (2) The extraction process of Salvia miltiorrhiza polysaccharides by ultrasonic method was 5g raw material, 100mL water solution, twice extraction, 30 min for each. (3) Antioxidtion tests indicated that with the concentration 0.05mg/mL of polysaccharides, the inhibition rate for superoxide anion radical could reach 1.28% and 13.54% for hydroxyl radical, respectively. And the reducing power was likely to increase with the increasing of polysaccharides concentration. 4. Conclusion: Ultrasonic method could effectively extract polysaccharide from Salvia miltiorrhiza. Antioxidation tests indicate that polysaccharides from Salvia miltiorrhiza have a certain removal effect on superoxide anion radical and hydroxyl radical.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Guangtao Jia
AU  - Hanqing Zhao
PY  - 2015/07
DA  - 2015/07
TI  - Extraction and Antioxidant Activity of Polysaccharide from Salvia miltiorrhiza
BT  - Proceedings of the International Conference on Chemical, Material and Food Engineering
PB  - Atlantis Press
SP  - 106
EP  - 109
SN  - 2352-5401
UR  - https://doi.org/10.2991/cmfe-15.2015.26
DO  - 10.2991/cmfe-15.2015.26
ID  - Jia2015/07
ER  -