Deletion of Lactate Dehydrogenase in Klebsiella Pneumoniae to Enhance 1,3-Propanediol Production

DOI

10.2991/cmes-15.2015.171How to use a DOI?

Keywords

K.pneumoniae, lactate dehydrogenase, 1,3-propanediol, batch fermentation.

Abstract

1,3-Propanediol (1,3-PD) is an important chemical intermediate in manufacturing polyethers, polyurethanes and polyesters. Production of 1,3-PD by microbial fermentation has attracted increasingly attention of researchers. In this study, deletion mutant of K.pneumoniae HD-1 constructed by knocking out lactate dehydrogenase (LDH) gene in our previous work was cultivated in fermentation medium utilizing glucose as carton source and original strain K.pneumoniae were simultaneously used as the control strain under absolutely the same conditions and their capability of 1,3-PD production were compared. The results showed that an increase of 19.98%, 20%, and 20.19% of 1,3-PD titer, productivity, and yield, respectively were obtained from K.pneumoniae HD-1. Obviously it can be inferred that the ability in the production of 1,3-PD of the K.pneumoniae HD-1was better than that of the K.pneumoniae, therefore it could be a promising strain for 1,3-PD production in the further study of industrialized production.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Guangbin Ye
AU  - Shanshan Sun
AU  - Liting Deng
AU  - Jingping Ge
AU  - Wenxiang Ping
PY  - 2015/04
DA  - 2015/04
TI  - Deletion of Lactate Dehydrogenase in Klebsiella Pneumoniae to Enhance 1,3-Propanediol Production
BT  - Proceedings of the 2nd International Conference on Civil, Materials and Environmental Sciences
PB  - Atlantis Press
SP  - 627
EP  - 629
SN  - 2352-5401
UR  - https://doi.org/10.2991/cmes-15.2015.171
DO  - 10.2991/cmes-15.2015.171
ID  - Ye2015/04
ER  -