Effects of Estrogen Receptor Alpha on Vascular Smooth Muscle Cell SM22

DOI

10.2991/cmes-15.2015.155How to use a DOI?

Keywords

SM22, Estrogen Receptor, Myocardin

Abstract

Myocardin and associated transcription factor (Myocardin-Related Transcription Factors, MRTFs) by serum response factor (SRF) together constitute the composition of molecular differentiation of muscle cell switch. This paper focuses on the vascular smooth muscle cells (vascular smooth muscle cells, VSMCs), breast cancer cell lines MCF-7 and African green monkey kidney cell line COS-7 as the research object, using cellular and molecular biological methods to study the transcription factor Myocardin and Estrogen receptor (ER ) in the regulation of differentiation of vascular smooth muscle cell function. The luciferase reporter assay in COS-7 and MCF-7 cell lines and VSMCs (Luciferase Report assay) method to study the ER alpha for Myocardin differentiation ability, the results showed that ER can inhibit SM22 Myocardin for transcription activation. At the same time, the expression of estradiol and tamoxifen treatment by transfection group for detection of SM22 and found that blocking the ER alpha for the inhibitory effect of Myocardin differentiation ability can be ER alpha antagonist tamoxi.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Mengmeng Qiang
PY  - 2015/04
DA  - 2015/04
TI  - Effects of Estrogen Receptor Alpha on Vascular Smooth Muscle Cell SM22
BT  - Proceedings of the 2nd International Conference on Civil, Materials and Environmental Sciences
PB  - Atlantis Press
SP  - 559
EP  - 562
SN  - 2352-5401
UR  - https://doi.org/10.2991/cmes-15.2015.155
DO  - 10.2991/cmes-15.2015.155
ID  - Qiang2015/04
ER  -