Optimization of Fermentation Parameters for Laccase Production by a Novel Deuteromycete Fungus Myrothecium Verrucaria NF-05 Using Response Surface Methodology
Authors
Dan Zhao, Yanyang Sun, Renpeng Du, Li Zhang, Jingping Ge
Corresponding Author
Dan Zhao
Available Online April 2015.
- DOI
- 10.2991/cmes-15.2015.118How to use a DOI?
- Keywords
- Laccase; Deuteromycete; Medium; Optimization; Response-surface methodology
- Abstract
To obtain the maximum production of laccase from a novel deuteromycete fungus Myrothecium verrucaria NF-05, optimization of fermentation parameters was performed. Central composite design and response surface analysis revealed the optimum concentration of glucose, CuSO4 and gallic acid to be 26.47 g L-1, 236.3 µM, and 138.4 µM, respectively. This optimization strategy led to the enhancement of laccase production from 12.00 to 19.94 U mL-1, 1.66-fold increase.
- Copyright
- © 2015, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Dan Zhao AU - Yanyang Sun AU - Renpeng Du AU - Li Zhang AU - Jingping Ge PY - 2015/04 DA - 2015/04 TI - Optimization of Fermentation Parameters for Laccase Production by a Novel Deuteromycete Fungus Myrothecium Verrucaria NF-05 Using Response Surface Methodology BT - Proceedings of the 2nd International Conference on Civil, Materials and Environmental Sciences PB - Atlantis Press SP - 425 EP - 427 SN - 2352-5401 UR - https://doi.org/10.2991/cmes-15.2015.118 DO - 10.2991/cmes-15.2015.118 ID - Zhao2015/04 ER -