The Influence of Myristicin Lost in Myristica Fragrans Volatile Oils to Antimicrobial Activity Against B. Subtilis, E. Coli and S. Aureus

DOI

10.2991/ahsr.k.200523.035How to use a DOI?

Keywords

myristica fragrans volatile oil, myristicin lost, antimicrobial activity

Abstract

Objectives: Myristica fragrans volatile oil has presented strong antimicrobial activity against a wide variety of bacteria. The antimicrobial activity would be expected to relate to the respective composition and their relative percentage, and possibly synergistic interaction between its components. M.fragrans volatile oil without myristicin have different relative percentage composition, so it is expected to have different antimicrobial activities. This study aimed to evaluate the correlation between different compound and percentage of M.fragrans volatile oil and myristicin to antimicrobial activities against different bacterial strains. Myristicin was isolated by fractional distillation method at 473.5 K, 25 mmHg. The M. fragrant volatile oil and myristicin screened for antimicrobial activity against B. Subtilis, E.Coli and S.Aureus using an agar diffusion technique. The concentration of each component range between 2,5-20%. The antimicrobial activity determined by measured zones of growth inhibition with 50 μl tetracycline disc as a positive control. The result showed that m. Fragrant volatile oils containing 31 compounds with myristicin content of 12.94%, while the essential oils of nutmeg without myristicin contain 16 compounds, both of them has same 5 major components i.e., α-pinene, sabinene, β-pinene, 4-terpineol and limonene, The volatile oil of M.fragrans exerting greatest inhibitory activity against S.Aureus followed by B.Subtilis and E.Coli with the zones of growth inhibition 18,3; 9,3; and 7,6mm respectively. The myristicin lost in M.fragrans volatile oil had lower antimicrobial activity against all bacterial strains. The zone of growth inhibition of fraction against S.Aureus, B.Subtilis, and E.Coli were 13,6; 8,6; and 7,3mm respectively.

Copyright

© 2020, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Anita Nilawati
AU  - Agatha Tyva Julian Atmajaningtyas
AU  - Hery Muhamad Ansory
PY  - 2020
DA  - 2020/06/08
TI  - The Influence of Myristicin Lost in Myristica Fragrans Volatile Oils to Antimicrobial Activity Against B. Subtilis, E. Coli and S. Aureus
BT  - The proceedings of the 2nd Bakti Tunas Husada-Health Science International Conference (BTH-HSIC 2019)
PB  - Atlantis Press
SP  - 143
EP  - 145
SN  - 2468-5739
UR  - https://doi.org/10.2991/ahsr.k.200523.035
DO  - 10.2991/ahsr.k.200523.035
ID  - Nilawati2020
ER  -