Evidences that Intake of L-valine May Affect the Lifespan-specific Local Gene Network Pattern in Caenorhabditis Elegans

DOI

10.2991/bst-17.2018.24How to use a DOI?

Keywords

L-valine, Longevity, Local gene network, Pattern, C. elegans.

Abstract

The purpose of this study is to preliminarily investigate whether branched-chain amino acids (BCAAs) prolong the lifespan of model organism Caenorhabditis elegans (C. elegans) through modifying the ageing-related local gene network (LGN) pattern. After synchronization adult C. elegans were fed with living E. coli strain OP50 on nematode growth medium (NGM) agar plates with or without BCAAs (L-valine, L-leucine, L-isoleucine). L-valine extended the average lifespan of C. elegans significantly at low concentration (0.1ž), but the other two amino acids affected lifespan slightly. Quantitative polymerase chain reaction (QPCR) was employed to quantify expression levels of thirteen genes that are closely related with BCAAs metabolic modules in the LGN. It was found that the thirteen genes' expression levels were differentially affected (some up-regulated, some down-regulated, and some had over 10-fold changes in the expression level) by intake of 0.1žVal, suggesting that the extension of lifespan through 0.1žVal was possibly through some uncharacterized lifespan-specific local gene network pattern change resulted from differential expression-level changes of a group of genes.

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Hong-Yuan Wang
AU  - Zhi-Zhou Zhang
PY  - 2018/02
DA  - 2018/02
TI  - Evidences that Intake of L-valine May Affect the Lifespan-specific Local Gene Network Pattern in Caenorhabditis Elegans
BT  - Proceedings of the 2017 2nd International Conference on Biological Sciences and Technology (BST 2017)
PB  - Atlantis Press
SP  - 161
EP  - 167
SN  - 2468-5747
UR  - https://doi.org/10.2991/bst-17.2018.24
DO  - 10.2991/bst-17.2018.24
ID  - Wang2018/02
ER  -