PCV Measurement for Biomass Assessment in Suspension Culture of Insect Cells

DOI

10.2991/bst-16.2016.50How to use a DOI?

Keywords

PCV measurement, Biomass assessment, Insect cell culture.

Abstract

In this study, we present a new approach for biomass assessment in insect cell culture using a disposable microcentrifuge tube, which is composed with an upper chamber for sample loading and a lower 5 L capillary for cell collection during centrifugation. The height of the cell pellet within the capillary is measured and expressed as the packed cell volume (PCV). PCV method showed an excellent correlation with cell counting measurement, but with lower average of the errors (3.3%) compared to manual cell counting (12.4%). PCV method was shown to be statistically reliable and more precise, rapid. And it also demonstrates the potential use of this method to monitor the changes in insect cell culture that can affect cell size, for example as seen under baculovirus infection.

Copyright

© 2016, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Dong-Chen Liu
AU  - Qiu-Ling Xie
PY  - 2016/01
DA  - 2016/01
TI  - PCV Measurement for Biomass Assessment in Suspension Culture of Insect Cells
BT  - Proceedings of the 2016 International Conference on Biological Sciences and Technology
PB  - Atlantis Press
SP  - 337
EP  - 342
SN  - 2468-5747
UR  - https://doi.org/10.2991/bst-16.2016.50
DO  - 10.2991/bst-16.2016.50
ID  - Liu2016/01
ER  -