The Study on Changes of NKT Cells Levels in Peripheral Blood of Children with Asthma

DOI

10.2991/bme-15.2015.26How to use a DOI?

Keywords

Asthma; Nature killer cell; T cell

Abstract

Abstract. Objective: To study the changes of natural killer T cells (NKT cells)in peripheral blood and the ability of NKT cells to proliferate in response. Methods: 80 bronchial asthma children and 40 healthy children were included. NKT cells and T cells were analyzed by flow cytometry instrument. The levels of IL-4, IFN- , IL-10 were tested in different period of NKT cell culture. Results: There were 46 cases were acute, 34 cases were remission. Between the three groups, CD4+, CD8+, CD4+/CD8+ and NKT cells percentage comparison difference were statistically significant (P<0.05). CD4 +, CD4 + / CD8 + percentage in acute episodes were higher than the remission group and normal group, but CD8 +, NKT cells percentage were lower than the remission group and normal group. The difference was statistically significant (P < 0.05). There were no statistically significant difference he remission group between remission group and normal group (P > 0.05). With the extension of incubation time, IL – 4 levels in three groups of children were significantly increased, and acute attack group were higher than that in remission group and normal group. The difference was statistically significant (P < 0.05). Serums IL - 10 in cultivating 48 h raised highest, cultivate 72h declined, and acute attack group were higher than that in remission group and normal group. The difference was statistically significant (P < 0.05). Conclusion: The pathogenesis of asthma may be related to the ratio and dysfunction of NKT cells.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Huilan Zhu
AU  - Xinxin Xin
AU  - Hongyan Zhang
PY  - 2015/09
DA  - 2015/09
TI  - The Study on Changes of NKT Cells Levels in Peripheral Blood of Children with Asthma
BT  - Proceedings of the 2015 International Forum on Bioinformatics and Medical Engineering
PB  - Atlantis Press
SP  - 143
EP  - 147
SN  - 2352-5401
UR  - https://doi.org/10.2991/bme-15.2015.26
DO  - 10.2991/bme-15.2015.26
ID  - Zhu2015/09
ER  -