Effects of Indian Sandalwood Essential Oil (Santalum album Linn) in Inhibiting the Growth of Biofilm Streptococcus Mutans ATCC 25175

DOI

10.2991/978-94-6463-284-2_91How to use a DOI?

Keywords

Dental Caries; Indian Sandalwood Essential Oil (Santalum album Linn); Streptococcus mutans; Biofilm

Abstract

Streptococcus mutans is a major cause of dental caries. The gold standard for antimicrobial mouthwash is chlorhexidine gluconate 0.2%. If used long term, chlorhexidine gluconate might induce an allergic response as well as tooth discoloration. The essential oil of Indian Sandalwood is a natural product with antimicrobial qualities and little adverse effects. The objective of this research was to see how Indian Sandalwood essential oil (Santalum album Linn) extract affected the biofilm formation of Streptococcus mutans ATCC 25175. Streptococcus mutans ATCC 25175 was cultivated in nutrient broth for 24 hours at 37 °C. Inoculating the culture into a 96-well plate resulted in the formation of biofilm. After that, the well plate was incubated for 24 hours. It was prepared essential oils of Indian Sandalwood with concentrations of 12.5%, 6.25%, 3.125%, and 1.56%. The biofilm assay technique was used to evaluate the biofilm for 15 minutes. Positive controls used 0.2% chlorhexidine gluconate. The optical density values at 595 nm wavelength were observed using a microplate reader to achieve the result. The optimal concentration of Indian Sandalwood essential oil is 1.56%, which can limit the formation of Streptococcus mutans ATCC 25175 biofilm. The ANOVA test reveals significant changes in each concentration group when compared to 0.2% chlorhexidine gluconate. The essential oil of Indian Sandalwood (Santalum album Linn) can suppress the biofilm formation of Streptococcus mutans ATCC 25175.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Velicia Ghina Nadhifa
AU  - Ciptadhi Tri Oka Binartha
PY  - 2023
DA  - 2023/11/09
TI  - Effects of Indian Sandalwood Essential Oil (Santalum album Linn) in Inhibiting the Growth of Biofilm Streptococcus Mutans ATCC 25175
BT  - Proceedings of the 4th Borobudur International Symposium on Science and Technology 2022 (BIS-STE 2022)
PB  - Atlantis Press
SP  - 869
EP  - 874
SN  - 2352-5401
UR  - https://doi.org/10.2991/978-94-6463-284-2_91
DO  - 10.2991/978-94-6463-284-2_91
ID  - Nadhifa2023
ER  -