Purification of Protease Enzymes from Wild Poinsettia (Euphorbia Heterophylla) Plant with Ammonium Sulphate

DOI

10.2991/978-94-6463-140-1_45How to use a DOI?

Keywords

ammonium sulphate; plant latex; protease activity; protease enzyme

Abstract

Protease enzymes are one of the essential enzyme groups that have been widely studied and have very high economic value. The study aimed to determine the optimum protease enzyme extraction method from the wild poinsettia (Euphorbia heterophylla) to produce the highest protease enzyme activity. It was a salting-out extraction method with adding ammonium sulphate salt at saturation levels of 50%, 60%, and 70%. The results showed that adding ammonium sulphate at a concentration of 50% is the most effective protease extraction from the latex of wild poinsettia. It showed the highest percentage yield (17.63 ± 4.74%) and the soluble protein content (4.76 ± 0.014%), while the protease enzyme activity was the lowest (2.9 x 103 ± 0.0142 unit/mg). The highest specific protease activity is achieved by extraction with 70% ammonium sulphate, i.e., 1.8 x 104 units/mg, at the saturation level of 50% and 60% ammonium sulphate; the specific activity of the protease wild poinsettia tended to be low. In conclusion, adding ammonium sulphate salt at saturation levels of 50%, 60%, and 70% showed the different characterization of protease enzymes from wild poinsettia plant latex.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - R. Amilia Destryana
AU  - Dyah Ayu Fajarianingtyas
AU  - Aryo Wibisono
AU  - Yuli Witono
AU  - Maria Belgis
AU  - Iwan Taruna
PY  - 2023
DA  - 2023/04/30
TI  - Purification of Protease Enzymes from Wild Poinsettia (Euphorbia Heterophylla) Plant with Ammonium Sulphate
BT  - Proceedings of the 2022 Brawijaya International Conference (BIC 2022)
PB  - Atlantis Press
SP  - 449
EP  - 456
SN  - 2352-5428
UR  - https://doi.org/10.2991/978-94-6463-140-1_45
DO  - 10.2991/978-94-6463-140-1_45
ID  - Destryana2023
ER  -